@article{fdi:010072075,
  title = {{A}ssessment of the gorilla gut virome in association with natural simian immunodeficiency virus infection},
  author = {{D}'{A}rc, {M}. and {F}urtado, {C}. and {S}iqueira, {J}. {D}. and {S}euanez, {H}. {N}. and {A}youba, {A}hidjo and {P}eeters, {M}artine and {S}oares, {M}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {S}imian immunodeficiency viruses ({SIV}s) of chimpanzees and gorillas from {C}entral {A}frica crossed the species barrier at least four times giving rise to human immunodeficiency virus type 1 ({HIV}-1) groups {M}, {N}, {O} and {P}. {T}he paradigm of non-pathogenic lentiviral infections has been challenged by observations of naturally infected chimpanzees with {SIV}cpz associated with a negative impact on their life span and reproduction, {CD}4(+) {T}-lymphocyte loss and lymphoid tissue destruction. {W}ith the advent and dissemination of new generation sequencing technologies, novel promising markers of immune deficiency have been explored in human and nonhuman primate species, showing changes in the microbiome (dysbiosis) that might be associated with pathogenic conditions. {T}he aim of the present study was to identify and compare enteric viromes of {SIV}gor-infected and uninfected gorillas using non-invasive sampling and ultradeep sequencing, and to assess the association of virome composition with potential {SIV}gor pathogenesis in their natural hosts. {R}esults: {W}e analyzed both {RNA} and {DNA} virus libraries of 23 fecal samples from 11 {SIV}gor-infected (two samples from one animal) and 11 uninfected western lowland gorillas from {C}ampo-{M}a'an {N}ational {P}ark ({CP}), in southwestern {C}ameroon. {T}hree bacteriophage families ({S}iphoviridae, {M}yoviridae and {P}odoviridae) represented 67.5 and 68% of the total annotated reads in {SIV}gor-infected and uninfected individuals, respectively. {C}onversely, mammalian viral families, such as {H}erpesviridae and {R}eoviridae, previously associated with gut-and several mammalian diseases were significantly more abundant (p < 0.003) in the {SIV}gor-infected group. {I}n the present study, we analyzed, for the first time, the enteric virome of gorillas and their association with {SIV}gor status. {T}his also provided the first evidence of association of specific mammalian viral families and {SIV}gor in a putative dysbiosis context. {C}onclusions: {O}ur results suggested that viromes might be potentially used as markers of lentiviral disease progression in wild gorilla populations. {T}he diverse mammalian viral families, herein described in {SIV}gor-infected gorillas, may play a pivotal role in a disease progression still unclear in these animals but already well characterized in pathogenic lentiviral infections in other organisms. {L}arger sample sets should be further explored to reduce intrinsic sampling variation.},
  keywords = {{G}orilla ; {SIV}gor ; {P}athogenesis ; {V}irome ; {D}ysbiosis ; {CAMEROUN}},
  booktitle = {},
  journal = {{R}etrovirology},
  volume = {15},
  numero = {},
  pages = {art. 19 [8 p.]},
  ISSN = {1742-4690},
  year = {2018},
  DOI = {10.1186/s12977-018-0402-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072075},
}