Combes Marie-Christine auteur aut IRD Joët Thierry auteur aut IRD Lashermes Philippe auteur aut IRD text journalArticle eng text/pdf born digital access Worldwide appreciated beverage, "Arabica" and "Robusta" coffee produced from Coffea arabica and C. canephora respectively, represents a high economical value product. Arabica coffee is generally sold at a higher price than Robusta coffee. As both intentional and accidental adulterations of Arabica with Robusta are frequent, a reliable, inexpensive, and high-throughput adulteration detection technology is required for the coffee industry. We developed a DNA-based authentication method that overcomes limitations of metabolic profiling of Robusta and Arabica coffees. The identification of Arabica and Robusta coffee species, as well as the quantification of their relative proportion in blends were performed by High Resolution Melting (HRM) analysis on green and roasted coffee products. For a more sensitive detection method, chloroplastic rather than nuclear genetic variations were targeted between Coffea species. By the developed method, the Coffea species of origin can be quickly differentiated and in case of adulteration, the percentage can be determined. specialized FRANCE CAMEROUN VIET NAM INDE BRESIL GUATEMALA 076 88 198-206 2018 0956-7135 https://www.documentation.ird.fr/hor/fdi:010071469 10.1016/j.foodcont.2018.01.014 0956-7135 [F B010071469] https://www.documentation.ird.fr/hor/fdi:010071469 https://www.documentation.ird.fr/intranet/publi/depot/2018-03-07/010071469.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2018-03-07 2023-07-11 fdi:010071469 fre