@article{fdi:010071409,
  title = {{C}omparison of different nucleic acid preparation methods to improve specific {HIV}-1 {RNA} isolation for viral load testing on dried blood spots},
  author = {{G}uichet, {E}. and {S}errano, {L}. and {L}aurent, {C}hristian and {E}ymard-{D}uvernay, {S}abrina and {K}uaban, {C}. and {V}idal, {L}aurent and {D}elaporte, {E}. and {N}gole, {E}. {M}. and {A}youba, {A}hidjo and {P}eeters, {M}artine},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n resource-limited countries ({RLC}s), {WHO} recommends {HIV} viral load ({VL}) on dried blood spots ({DBS}) for antiretroviral therapy ({ART}) monitoring of patients living in non-urban settings where plasma {VL} is not available. {I}n order to reduce the impact of proviral {DNA} interference, leading to false positive results in samples with low plasma {VL}, we compared three different nucleic acid preparation methods with the {N}ucli{S}ens ({B}iomerieux) extraction, known for its high recovery of nucleic acids on {DBS}. {P}aired plasma-{DBS} samples (n = 151) with predominantly low plasma {VL} (<= 10,000 copies/ml; 74%) were used. {A}t the threshold of 1,000 copies/ml on {DBS}, 51% and 10% were misclassified as false positives or false negatives, respectively with {N}ucli{S}ens, versus 41% and 20% with m2000sp ({A}bbott), described as more specific for {RNA} recovery. {DN}ase treatments of nucleic acid extracts and free virus elution ({FVE}) protocol before nucleic acid extraction, reduced the proportion of false positives to 0% and 19%, but increased the proportion of false negatives to 40% and 73%. {M}ore efforts are thus still needed to improve performance of {VL} assays on {DBS} to monitor patients on {ART} in {RLC}s and allow timely switch to more costly second or third line {ART} regimes.},
  keywords = {{D}ried blood spot ; {HIV}-1 ; {R}esource limited countries ; {RNA} extraction ; {V}iral load ; {PAYS} {EN} {DEVELOPPEMENT}},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {251},
  numero = {},
  pages = {75--79},
  ISSN = {0166-0934},
  year = {2018},
  DOI = {10.1016/j.jviromet.2017.10.014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071409},
}