Eldering M. auteur aut IRD Bompard A. auteur aut IRD Miura K. auteur aut IRD Stone W. auteur aut IRD Morlais Isabelle auteur aut IRD Cohuet Anna auteur aut IRD van Gemert G. J. auteur aut IRD Brock P. M. auteur aut IRD Rijpma S. R. auteur aut IRD van de Vegte-Bolmer M. auteur aut IRD Graumans W. auteur aut IRD Siebelink-Stoter R. auteur aut IRD Da D. F. auteur aut IRD Long C. A. auteur aut IRD Morin M. J. auteur aut IRD Sauerwein R. W. auteur aut IRD Churcher T. S. auteur aut IRD Bousema T. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Background: With the increasing interest in vaccines to interrupt malaria transmission, there is a demand for harmonization of current methods to assess Plasmodium transmission in laboratory settings. Potential vaccine candidates are currently tested in the standard membrane feeding assay (SMFA) that commonly relies on Anopheles stephensi mosquitoes. Other mosquito species including Anopheles gambiae are the dominant malaria vectors for Plasmodium falciparum in sub-Saharan Africa. Methods: Using human serum and monoclonal pre-fertilization (anti-Pfs48/45) and post-fertilization (anti-Pfs25) antibodies known to effectively inhibit sporogony, we directly compared SMFA based estimates of transmissionreducing activity (TRA) for An. stephensi and An. gambiae mosquitoes. Results: In the absence of transmission-reducing antibodies, average numbers of oocysts were similar between An. gambiae and An. stephensi. Antibody-mediated TRA was strongly correlated between both mosquito species, and absolute TRA estimates for pre-fertilisation monoclonal antibodies (mAb) showed no significant difference between the two species. TRA estimates for IgG of naturally exposed individuals and partially effective concentrations of anti-Pfs25 mAb were higher for An. stephensi than for An. gambiae. Conclusion: Our findings support the use of An. stephensi in the SMFA for target prioritization. As a vaccine moves through product development, better estimates of TRA and transmission- blocking activity (TBA) may need to be obtained in epidemiologically relevant parasite-species combination. specialized Malaria Anopheles Transmission Vaccine Immunity AFRIQUE SUBSAHARIENNE 052 050 10 art. 489 [10 p.] 2017 1756-3305 https://www.documentation.ird.fr/hor/fdi:010071316 10.1186/s13071-017-2414-z 1756-3305 [F B010071316] https://www.documentation.ird.fr/hor/fdi:010071316 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-11/010071316.pdf IRD - Base Horizon / Pleins textes 2017-12-04 2023-07-11 fdi:010071316 fre