@article{fdi:010071316,
  title = {{C}omparative assessment of {A}n. gambiae and {A}n. stephensi mosquitoes to determine transmission-reducing activity of antibodies against {P}. falciparum sexual stage antigens},
  author = {{E}ldering, {M}. and {B}ompard, {A}. and {M}iura, {K}. and {S}tone, {W}. and {M}orlais, {I}sabelle and {C}ohuet, {A}nna and van {G}emert, {G}. {J}. and {B}rock, {P}. {M}. and {R}ijpma, {S}. {R}. and van de {V}egte-{B}olmer, {M}. and {G}raumans, {W}. and {S}iebelink-{S}toter, {R}. and {D}a, {D}. {F}. and {L}ong, {C}. {A}. and {M}orin, {M}. {J}. and {S}auerwein, {R}. {W}. and {C}hurcher, {T}. {S}. and {B}ousema, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {W}ith the increasing interest in vaccines to interrupt malaria transmission, there is a demand for harmonization of current methods to assess {P}lasmodium transmission in laboratory settings. {P}otential vaccine candidates are currently tested in the standard membrane feeding assay ({SMFA}) that commonly relies on {A}nopheles stephensi mosquitoes. {O}ther mosquito species including {A}nopheles gambiae are the dominant malaria vectors for {P}lasmodium falciparum in sub-{S}aharan {A}frica. {M}ethods: {U}sing human serum and monoclonal pre-fertilization (anti-{P}fs48/45) and post-fertilization (anti-{P}fs25) antibodies known to effectively inhibit sporogony, we directly compared {SMFA} based estimates of transmissionreducing activity ({TRA}) for {A}n. stephensi and {A}n. gambiae mosquitoes. {R}esults: {I}n the absence of transmission-reducing antibodies, average numbers of oocysts were similar between {A}n. gambiae and {A}n. stephensi. {A}ntibody-mediated {TRA} was strongly correlated between both mosquito species, and absolute {TRA} estimates for pre-fertilisation monoclonal antibodies (m{A}b) showed no significant difference between the two species. {TRA} estimates for {I}g{G} of naturally exposed individuals and partially effective concentrations of anti-{P}fs25 m{A}b were higher for {A}n. stephensi than for {A}n. gambiae. {C}onclusion: {O}ur findings support the use of {A}n. stephensi in the {SMFA} for target prioritization. {A}s a vaccine moves through product development, better estimates of {TRA} and transmission- blocking activity ({TBA}) may need to be obtained in epidemiologically relevant parasite-species combination.},
  keywords = {{M}alaria ; {A}nopheles ; {T}ransmission ; {V}accine ; {I}mmunity ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {10},
  numero = {},
  pages = {art. 489 [10 p.]},
  ISSN = {1756-3305},
  year = {2017},
  DOI = {10.1186/s13071-017-2414-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071316},
}