Le Coupanec A. auteur aut IRD Tchankouo-Nguetcheu S. auteur aut IRD Roux P. auteur aut IRD Khun H. auteur aut IRD Huerre M. auteur aut IRD Morales-Vargas R. auteur aut IRD Enguehard M. auteur aut IRD Lavillette D. auteur aut IRD Missé Dorothée auteur aut IRD Choumet V. auteur aut IRD text journalArticle eng text/pdf born digital access Arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. Chikungunya-affected areas often overlap with dengue-endemic areas. Concurrent dengue virus (DENV) and chikungunya virus (CHIKV) infections have been detected in travelers returning from regions of endemicity. CHIKV and DENV co-infected Aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosquitoes. We thus aimed to study the pathogen-pathogen interaction involved in these co-infections in DENV/CHIKV co-infected Aedes aegypti mosquitoes. In mono-infections, we detected CHIKV antigens as early as 4 days post-virus exposure in both the midgut (MG) and salivary gland (SG), whereas we detected DENV serotype 2 (DENV-2) antigens from day 5 post-virus exposure in MG and day 10 post-virus exposure in SG. Identical infection rates were observed for singly and co-infected mosquitoes, and facilitation of the replication of both viruses at various times post-viral exposure. We observed a higher replication for DENV-2 in SG of co-infected mosquitoes. We showed that mixed CHIKV and DENV infection facilitated viral replication in Ae. aegypti. The outcome of these mixed infections must be further studied to increase our understanding of pathogen-pathogen interactions in host cells. specialized Aedes aegypti arbovirus chikungunya dengue co-infection 052 020 18 8 art. 1708 [17 p.] 2017 1422-0067 https://www.documentation.ird.fr/hor/fdi:010071025 10.3390/ijms18081708 1422-0067 [F B010071025] https://www.documentation.ird.fr/hor/fdi:010071025 https://www.documentation.ird.fr/intranet/publi/2017/10/010071025.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2017-10-31 2023-07-11 fdi:010071025 fre