@article{fdi:010070834,
  title = {{T}ranscriptional profiling of midguts prepared from {T}rypanosoma/{T}. congolense-positive {G}lossina palpalis palpalis collected from two distinct {C}ameroonian foci : coordinated signatures of the {M}idguts' remodeling as {T}.congolense-supportive niches},
  author = {{N}goune, {J}. {M}. {T}. and {N}jiokou, {F}. and {L}oriod, {B}. and {K}ame-{N}gasse, {G}. and {F}ernandez-{N}unez, {N}. and {R}ioualen, {C}. and van {H}elden, {J}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ur previous transcriptomic analysis of {G}lossina palpalis gambiensis experimentally infected or not with {T}rypanosoma brucei gambiense aimed to detect differentially expressed genes ({DEG}s) associated with infection. {S}pecifically, we selected candidate genes governing tsetse fly vector competence that could be used in the context of an anti-vector strategy, to control human and/or animal trypanosomiasis. {T}he present study aimed to verify whether gene expression in field tsetse flies ({G}. p. palpalis) is modified in response to natural infection by trypanosomes ({T}. congolense), as reported when insectary-raised flies ({G}. p. gambiensis) are experimentally infected with {T}. b. gambiense. {T}his was achieved using the {RNA}-seq approach, which identified 524 {DEG}s in infected vs. non-infected tsetse flies, including 285 downregulated genes and 239 upregulated genes (identified using {DES}eq2). {S}everal of these genes were highly differentially expressed, with log2 fold change values in the vicinity of either +40 or -40. {D}ownregulated genes were primarily involved in transcription/translation processes, whereas encoded upregulated genes governed amino acid and nucleotide biosynthesis pathways. {T}he {B}io{C}yc metabolic pathways associated with infection also revealed that downregulated genes were mainly involved in fly immunity processes. {I}mportantly, our study demonstrates that data on the molecular cross-talk between the host and the parasite (as well as the always present fly microbiome) recorded from an experimental biological model has a counterpart in field flies, which in turn validates the use of experimental host/parasite couples.},
  keywords = {field tsetse fly ; {C}ameroonian foci ; {RNA}seq ; vector control ; trypanosomiasis ; {CAMEROUN}},
  booktitle = {},
  journal = {{F}rontiers in {I}mmunology},
  volume = {8},
  numero = {},
  pages = {art. 876 [14 p.]},
  ISSN = {1664-3224},
  year = {2017},
  DOI = {10.3389/fimmu.2017.00876},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070834},
}