Williams-Wietzikoski C. A. auteur aut IRD So I. D. auteur aut IRD Bull M. E. auteur aut IRD Samleerat T. auteur aut IRD Pathanapitoon K. auteur aut IRD Kunavisarut P. auteur aut IRD Kongyai N. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Frenkel L. M. auteur aut IRD Sirirungsi W. auteur aut IRD text journalArticle eng text/pdf born digital access Objective: To analyze and compare HIV-1 env sequences from the eye to those from the blood of individuals with uveitis attributed to HIV with the goal of gaining insight into the pathogenesis of HIV-associated eye disease. Design: A prospective case series of five HIV-infected antiretroviral-naive individuals with uveitis negative for other pathogens. Methods: RNA from blood plasma and ocular aqueous humor was reverse transcribed using random hexamers. HIV env C2-V5 (HXB2: 6990-7668) sequences were generated by single-genome amplification using nested polymerase chain reaction followed by bidirectional Sanger sequencing. Sequence analyses by Geneious, Geno2Pheno, N-GLYCOSITE, DIVEIN, and HyPhy evaluated relationships between HIV in plasma and aqueous humor. Results: A median of 20 (range: 13-22) plasma and 15 (range: 9-18) aqueous humor sequences were generated from each individual. The frequencies of sequences with predicted-N-linked-glycosylation sites and C-X-C chemokine receptor type 4 were comparable in aqueous humor and plasma of all five patients. Aqueous humor sequences had lower median genetic diversity compared with plasma across all patients, but similar divergence, in four of five patients. Aqueous humor HIV sequences were compartmentalized from plasma across subjects by Critchlow correlation coefficient, Slatkin and Maddison, nearest-neighbor statistic, and Fixation index. Conclusion: Among antiretroviral-naive individuals with uveitis attributed to HIV, the universal compartmentalization and decreased diversity of eye compared with blood sequences suggests time-limited passage of a small subset of variants from each patient's viral population into the eye tissues, followed by limited immune selection despite the inflammatory uveitis. specialized aqueous humor C-X-C chemokine receptor type 4 and C-C chemokine receptor type 5 coreceptor usage HIV-1 predicted N-linked glycosylation sites single-genome amplification uveitis virus compartmentalization THAILANDE 052 31 13 1825-1830 2017 0269-9370 https://www.documentation.ird.fr/hor/fdi:010070390 10.1097/qad.0000000000001550 0269-9370 [F B010070390] https://www.documentation.ird.fr/hor/fdi:010070390 https://www.documentation.ird.fr/intranet/publi/2017/08/010070390.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2017-09-05 2018-01-02 fdi:010070390 fre