Dahmani M. auteur aut IRD Davoust B. auteur aut IRD Tahir D. auteur aut IRD Raoult D. auteur aut IRD Fenollar F. auteur aut IRD Mediannikov Oleg auteur aut IRD text journalArticle eng text/pdf reformatted digital access Backgrounds: Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia. The aim of this study was to identify and estimate the prevalence of Anaplasmataceae species infecting domestic animals and ticks in Corsica. Methods: In this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in Corsica. Quantitative real-time PCR screening and genetic characterisation of Anaplasmataceae bacteria were based on the 23S rRNA, rpoB and groEl genes. Results: Two tick species were collected in the present study: Rhipicephalus bursa (118) and Hyalomma marginatum marginatum (5). Molecular investigation showed that 32.1% (147/458) of blood samples were positive for Anaplasmataceae infection. Anaplasma ovis was identified in 42.3% (93/220) of sheep. Anaplasma marginale was amplified from 100% (12/12) of cattle and two R. bursa (2/123). Several potentially new species were also identified: Anaplasma cf. ovis, "Candidatus Anaplasma corsicanum", "Candidatus Anaplasma mediterraneum" were amplified from 17.3% (38/220) of sheep, and Anaplasma sp. marginale-like was amplified from 80% (4/5) of goats. Finally, one R. bursa tick was found to harbour the DNA of E. canis. All samples from horses and dogs were negative for Anaplasmataceae infection. Conclusions: To our knowledge, this study is the first epidemiological survey on Anaplasmataceae species infecting animals and ticks in Corsica and contributes toward the identification of current Anaplasmataceae species circulating in Corsica. specialized Corsica Island Animals Ticks Anaplasma ovis Anaplasma marginale Anaplasma sp. Ehrlichia canis FRANCE CORSE 052 080 10 art. 302 [12 p.] 2017 1756-3305 https://www.documentation.ird.fr/hor/fdi:010070245 10.1186/s13071-017-2233-2 1756-3305 [F B010070245] https://www.documentation.ird.fr/hor/fdi:010070245 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-07/010070245.pdf IRD - Base Horizon / Pleins textes 2017-07-26 2023-07-11 fdi:010070245 fre