Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Frontiers in Microbiology art. 748 [7 p.] Vibrio cholerae O1 virulence antibioresistance SXT element Senegal SENEGAL 2017 fdi:010070052 ENG Frontiers in Microbiology 1664-302X ISI:000401571100001 10.3389/fmicb.2017.00748 https://www.documentation.ird.fr/hor/fdi:010070052 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-06/010070052.pdf 8 Horizon (IRD) Vibrio cholerae O1 is the causative agent of cholera with classical and El Tor, two well-established biotypes. In last 20 years, hybrid strains of classical and El Tor and variant El Tor which carry classical ctxB have emerged worldwide. In 2004-2005, Senegal experienced major cholera epidemic with a number of cases totalling more than 31719 with approximately 458 fatal outcomes (CFR, 1.44%). In this retrospective study, fifty isolates out of a total of 403 V. cholerae biotype El Tor serovar Ogawa isolates from all areas in Senegal during the 2004-2005 cholera outbreak were randomly selected. Isolates were characterized using phenotypic and genotypic methods. The analysis of antibiotic resistance patterns revealed the predominance of the S-Su-TCY-Tsu phenotype (90% of isolates). The molecular characterization of antibiotic resistance revealed the presence of the SXT element, a self-transmissible chromosomally integrating element in all isolates. Most of V. cholerae isolates had an intact virulence cassette (86%) (ctx, zot, ace genes). All isolates tested gave amplification with primers for classical CT, and 10/50 (20%) of isolates carried classical and El Tor ctxB. The study reveals the presence of atypical V. cholerae O1 El Tor during cholera outbreak in Senegal in 2004-2005. 052 ; 050 UR224 Sénégal