@article{fdi:010070039,
  title = {{T}ranscriptomic and histological responses of {A}frican rice ({O}ryza glaberrima) to {M}eloidogyne graminicola provide new insights into root-knot nematode resistance in monocots},
  author = {{P}etitot, {A}nne-{S}ophie and {K}yndt, {T}. and {H}aidar, {R}. and {D}ereeper, {A}lexis and {C}ollin, {M}yriam and {E}ngler, {J}. {D}. and {G}heysen, {G}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and {A}ims {T}he root-knot nematode {M}eloidogyne graminicola is responsible for production losses in rice ({O}ryza sativa) in {A}sia and {L}atin {A}merica. {T}he accession {TOG}5681 of {A}frican rice, {O}. glaberrima, presents improved resistance to several biotic and abiotic factors, including nematodes. {T}he aim of this study was to assess the cytological and molecular mechanisms underlying nematode resistance in this accession. {M}ethods {P}enetration and development in {M}. graminicola in {TOG}5681 and the susceptible {O}. sativa genotype '{N}ipponbare' were compared by microscopic observation of infected roots and histological analysis of galls. {I}n parallel, host molecular responses to {M}. graminicola were assessed by root transcriptome profiling at 2, 4 and 8 d postinfection (dpi). {S}pecific treatments with hormone inhibitors were conducted in {TOG}5681 to assess the impact of the jasmonic acid and salicylic acid pathways on nematode penetration and reproduction. {K}ey {R}esults {P}enetration and development of {M}. graminicola juveniles were reduced in the resistant {TOG}5681 in comparison with the susceptible accession, with degeneration of giant cells observed in the resistant genotype from 15 dpi onwards. {T}ranscriptome changes were observed as early as 2 dpi, with genes predicted to be involved in defence responses, phenylpropanoid and hormone pathways strongly induced in {TOG}5681, in contrast to '{N}ipponbare'. {N}o specific hormonal pathway could be identified as the major determinant of resistance in the ricenematode incompatible interaction. {C}andidate genes proposed as involved in resistance to {M}. graminicola in {TOG}5681 were identified based on their expression pattern and quantitative trait locus ({QTL}) position, including chalcone synthase, isoflavone reductase, phenylalanine ammonia lyase, {WRKY}62 transcription factor, thionin, stripe rust resistance protein, thaumatins and {ATP}ase3. {C}onclusions {T}his study provides a novel set of candidate genes for {O}. glaberrima resistance to nematodes and highlights the rice-{M}. graminicola pathosystem as a model to study plant-nematode incompatible interactions.},
  keywords = {{O}ryza sativa ; {O}ryza glaberrima ; {M}eloidogyne graminicola ; root-knot nematode ; resistance responses ; {RNA}-{S}eq ; transcriptomics ; histology ; hormones ; {QTL} ; differentially expressed genes ; monocots ; {AFRIQUE}},
  booktitle = {},
  journal = {{A}nnals of {B}otany},
  volume = {119},
  numero = {5},
  pages = {885--899},
  ISSN = {0305-7364},
  year = {2017},
  DOI = {10.1093/aob/mcw256},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070039},
}