%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Drancourt, M.
%A Aboudharam, G.
%A Croce, O.
%A Armougom, Fabrice
%A Robert, C.
%A Raoult, D.
%T Dental pulp as a source of low-contaminated DNA
%D 2017
%L fdi:010069964
%G ENG
%J Microbial Pathogenesis
%@ 0882-4010
%K Dental pulp ; Contamination ; Metagenomics ; Forensic sciences ; Paleomicrobiology ; Paleogenetics
%M ISI:000398650700010
%P 63-67
%R 10.1016/j.micpath.2017.02.007
%U https://www.documentation.ird.fr/hor/fdi:010069964
%> https://www.documentation.ird.fr/intranet/publi/2017/05/010069964.pdf
%V 105
%W Horizon (IRD)
%X The in-laboratory contamination of the ancient samples hinders the result interpretation of the investigations in the field of paleomicrobiology. We had promoted the dental pulp as a sample that limits the risks of in-laboratory contamination of the ancient material. In this work, we measured the contamination of the dental pulp manipulated according to paleomicrobiology protocol, used as a source of a total DNA for metagenomics. First, total DNA extracted from two dog canines was sequenced using next generation sequencing. This yielded a total of 487,828 trimmed reads with a length of 227 +/- 35 bp. Sequence analysis of the final dataset using Blast algorithm search and stringent thresholds for sequence identity and coverage against a database including both Canis lupus familiaris and Homo sapiens complete genomes showed that 95% of reads were assigned to C familiaris whereas 0.03% was assigned to H. sapiens. In a second step, two teeth collected from two 12th century mammals were manipulated following the same protocol. A total of 13,890 trimmed reads with a 157 +/- 67 bp length yielded 0-0.35% reads assigned to H. sapiens. This study indicates that the dental pulp is a useful for detecting the significant nucleic sequences in both modern and ancient samples.
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