@article{fdi:010069964,
  title = {{D}ental pulp as a source of low-contaminated {DNA}},
  author = {{D}rancourt, {M}. and {A}boudharam, {G}. and {C}roce, {O}. and {A}rmougom, {F}abrice and {R}obert, {C}. and {R}aoult, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he in-laboratory contamination of the ancient samples hinders the result interpretation of the investigations in the field of paleomicrobiology. {W}e had promoted the dental pulp as a sample that limits the risks of in-laboratory contamination of the ancient material. {I}n this work, we measured the contamination of the dental pulp manipulated according to paleomicrobiology protocol, used as a source of a total {DNA} for metagenomics. {F}irst, total {DNA} extracted from two dog canines was sequenced using next generation sequencing. {T}his yielded a total of 487,828 trimmed reads with a length of 227 +/- 35 bp. {S}equence analysis of the final dataset using {B}last algorithm search and stringent thresholds for sequence identity and coverage against a database including both {C}anis lupus familiaris and {H}omo sapiens complete genomes showed that 95% of reads were assigned to {C} familiaris whereas 0.03% was assigned to {H}. sapiens. {I}n a second step, two teeth collected from two 12th century mammals were manipulated following the same protocol. {A} total of 13,890 trimmed reads with a 157 +/- 67 bp length yielded 0-0.35% reads assigned to {H}. sapiens. {T}his study indicates that the dental pulp is a useful for detecting the significant nucleic sequences in both modern and ancient samples.},
  keywords = {{D}ental pulp ; {C}ontamination ; {M}etagenomics ; {F}orensic sciences ; {P}aleomicrobiology ; {P}aleogenetics},
  booktitle = {},
  journal = {{M}icrobial {P}athogenesis},
  volume = {105},
  numero = {},
  pages = {63--67},
  ISSN = {0882-4010},
  year = {2017},
  DOI = {10.1016/j.micpath.2017.02.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069964},
}