Broutin V. auteur aut IRD Banuls Anne-Laure auteur aut IRD Aubry A. auteur aut IRD Keck N. auteur aut IRD Choisy Marc auteur aut IRD Bernardet J. F. auteur aut IRD Michel C. auteur aut IRD Raymond J. C. auteur aut IRD Libert C. auteur aut IRD Barnaud A. auteur aut IRD Stragier P. auteur aut IRD Portaels F. auteur aut IRD Terru D. auteur aut IRD Belon C. auteur aut IRD Dereure O. auteur aut IRD Gutierrez C. auteur aut IRD Boschiroli M. L. auteur aut IRD Van De Perre P. auteur aut IRD Cambau E. auteur aut IRD Godreuil S. auteur aut IRD text journalArticle eng text/pdf born digital access Mycobacterium marinum causes a systemic tuberculosis-like disease in fish and skin infections in humans that can spread to deeper structures, resulting in tenosynovitis, arthritis, and osteomyelitis. However, little information is available concerning (i) the intraspecific genetic diversity of M. marinum isolated from humans and animals; (ii) M. marinum genotype circulation in the different ecosystems, and (iii) the link between M. marinum genetic diversity and hosts (humans and fish). Here, we conducted a genetic study on 89 M. marinum isolates from humans (n = 68) and fish (n = 21) by using mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing. The results show that the M. marinum population is genetically structured not only according to the host but also according to the ecosystem as well as to tissue tropism in humans. This suggests the existence of different genetic pools in the function of the biological and ecological compartments. Moreover, the presence of only certain M. marinum genotypes in humans suggests a different zoonotic potential of the M. marinum genotypes. Considering that the infection is linked to aquarium activity, a significant genetic difference was also detected when the human tissue tropism of M. marinum was taken into consideration, with a higher genetic polymorphism in strains isolated from patients with cutaneous forms than from individuals with deeper-structure infection. It appears that only few genotypes can produce deeper infections in humans, suggesting that the immune system might play a filtering role. specialized 052 050 50 11 3627-3634 2012 0095-1137 https://www.documentation.ird.fr/hor/fdi:010069611 0095-1137 [F B010069611] https://www.documentation.ird.fr/hor/fdi:010069611 https://www.documentation.ird.fr/intranet/publi/depot/2017-03-27/010069611.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-11-30 2017-11-02 fdi:010069611 fre