Bournaud C. auteur aut IRD Moulin Lionel auteur aut IRD Cnockaert M. auteur aut IRD de Faria S. auteur aut IRD Prin Y. auteur aut IRD Severac D. auteur aut IRD Vandamme P. auteur aut IRD text journalArticle eng text/pdf born digital access During a survey of root-nodulating symbionts of Mimosoid species in the south-east region of Brazil, eight Paraburkholderia isolates were obtained from nodules of the legume species Piptadenia gonoacantha, either from the field or following a soil trapping method with the same plant host. 16S rRNA gene as well as recA and gyrB phylogenetic markers placed these strains in two new clades within the genus Burkholderia sensu lato. DNA-DNA hybridization values and analyses of average nucleotide identities of the whole genome sequence of selected strains in each clade (STM 7183 and STM 7296) showed that the two clades represented novel species of the genus Paraburkholderia. All eight isolates were further characterized using DNA base content determination, chemotaxonomic and biochemical profiling and symbiotic properties, which allowed to distinguish the novel species from known diazotrophic species of the genus Paraburkholderia. Based on genomic and phenotypic data, the names Paraburkholderia piptadeniae sp. nov. with type strain STM 7183(T) (= DSM 101189(T) = LMG 29163(T)) and Paraburkholderia ribeironis sp. nov. with type strain STM 7296(T) (= DSM 101188(T) = LMG 29351(T)) are proposed. specialized Burkholderia rhizobia legumes mimosoids symbiosis genomes BRESIL 084 67 2 432-440 2017 1466-5026 https://www.documentation.ird.fr/hor/fdi:010069466 10.1099/ijsem.0.001648 1466-5026 [F B010069466] https://www.documentation.ird.fr/hor/fdi:010069466 https://www.documentation.ird.fr/intranet/publi/2017/04/010069466.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2017-05-09 2024-05-13 fdi:010069466 fre