%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Maïga, O.
%A Djimde, A. A.
%A Hubert, V.
%A Renard, E.
%A Aubouy, Agnès
%A Kironde, F.
%A Nsimba, B.
%A Koram, K.
%A Doumbo, O. K.
%A Bras, J.
%A Clain, J.
%T A shared Asian origin of the triple-mutant dhfr allele in Plasmodium falciparum from sites across Africa
%D 2007
%L fdi:010069213
%G ENG
%J Journal of Infectious Diseases
%@ 0022-1899
%K BENIN ; CAMEROUN ; CONGO ; COTE D'IVOIRE ; GABON ; GUINEE ; MALI ; SENEGAL ; OUGANDA ; COMORES
%M CC:0002469871-0024
%N 1
%P 165-172
%R 10.1086/518512
%U https://www.documentation.ird.fr/hor/fdi:010069213
%V 196
%W Horizon (IRD)
%X Background. Usefulness of sulfadoxine-pyrimethamine as first-line therapy for uncomplicated Plasmodium falciparum malaria and intermittent preventive treatment in pregnancy throughout sub-Saharan Africa is compromised by the spread of dhfr alleles associated with pyrimethamine resistance. A predominant haplotype associated with the N51I+C59R+S108N triple-mutant dhfr allele has been reported recently in 4 African countries. A more comprehensive picture of the evolution of this mutant allele in Africa is lacking. Methods. Seventy-five P. falciparum isolates carrying the wild-type dhfr allele and 204 carrying the triple-mutant dhfr allele from 11 African countries were selected. The genetic diversity of the chromosomes bearing these alleles was analyzed with 4 microsatellite markers closely linked to the dhfr gene. Results. Seventy-three different 4-locus haplotypes carrying the wild-type dhfr allele were found. By contrast, 175 ( 85%) of 204 isolates carrying the triple-mutant dhfr allele shared a unique haplotype, identical to the one identified in Thailand. For the remaining triple-mutant isolates and one isolate with the quadruple-mutant dhfr allele ( N51I+C59R+S108N+I164L), haplotypes were closely related to the predominant haplotype by mutation or recombination. Conclusions. Migration of parasites carrying an ancestral triple-mutant dhfr allele drives the spread of dhfr alleles associated with pyrimethamine resistance throughout West and Central Africa.
%$ 052