@article{fdi:010069207,
  title = {{C}arboxypeptidases {B} of {A}nopheles gambiae as targets for a {P}lasmodium falciparum transmission-blocking vaccine},
  author = {{L}avazec, {C}. and {B}oudin, {C}hristian and {L}acroix, {R}. and {B}onnet, {S}. and {D}iop, {A}bdoulaye and {T}hiberge, {S}. and {B}oisson, {B}. and {T}ahar, {R}achida and {B}ourgouin, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}nopheles gambiae is the major {A}frican vector of {P}lasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in {A}frica. {S}everal strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines ({TBV}s), which induce in the vertebrate host the production of antibodies that inhibit parasite development in the mosquito midgut. {S}o far, the most promising components of a {TBV} are parasite-derived antigens, although targeting critical mosquito components might also successfully block development of the parasite in its vector. {W}e previously identified {A}. gambiae genes whose expression was modified in {P}. falciparum-infected mosquitoes, including one midgut carboxypeptidase gene, cpb{A}g1. {H}ere we show that {P}. falciparum up-regulates the expression of cpb{A}g1 and of a second midgut carboxypeptidase gene, cpb{A}g2, and that this up-regulation correlates with an increased carboxypeptidase {B} ({CPB}) activity at a time when parasites establish infection in the mosquito midgut. {T}he addition of antibodies directed against {CPBA}g1 to a {P}.falciparum-containing blood meal inhibited {CPB} activity and blocked parasite development in the mosquito midgut. {F}urthermore, the development of the rodent parasite {P}lasmodium berghei was significantly reduced in mosquitoes fed on infected mice that had been immunized with recombinant {CPBA}g1. {L}astly, mosquitoes fed on anti-{CPBA}g1 antibodies exhibited reduced reproductive capacity, a secondary effect of a {CPB}-based {TBV} that could likely contribute to reducing {P}lasmodium transmission. {T}hese results indicate that {A}. gambiae {CPB}s could constitute targets for a {TBV} that is based upon mosquito molecules.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{I}nfection and {I}mmunity},
  volume = {75},
  numero = {4},
  pages = {1635--1642},
  ISSN = {0019-9567},
  year = {2007},
  DOI = {10.1128/{IAI}.00864-06},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069207},
}