%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Zghal, R. Z.
%A Elleuch, J.
%A Ben Ali, M.
%A Darriet, Frédéric
%A Rebai, A.
%A Chandre, Fabrice
%A Jaoua, S.
%A Tounsi, S.
%T Towards novel Cry toxins with enhanced toxicity/broader : a new chimeric Cry4Ba / Cry1Ac toxin [plus erratum]
%D 2017
%L fdi:010068859
%G ENG
%J Applied Microbiology and Biotechnology
%@ 0175-7598
%K Bacillus thuringiensis kurstaki ; Chimerical protein Cry(4Ba-1Ac) ; Toxicity improvement to Culex pipiens
%M ISI:000391365800009
%N 1
%P 113-122 [+ erratum, vol. 101, no 14,  5949]
%R 10.1007/s00253-016-7766-3
%U https://www.documentation.ird.fr/hor/fdi:010068859
%> https://www.documentation.ird.fr/intranet/publi/depot/2017-08-25/010068859.pdf
%V 101
%W Horizon (IRD)
%X Attempts have been made to express or to merge different Cry proteins in order to enhance toxic effects against various insects. Cry1A proteins of Bacillus thuringiensis form a typical bipyramidal parasporal crystal and their protoxins contain a highly conserved C-terminal region. A chimerical gene, called cry(4Ba-1Ac), formed by a fusion of the N-terminus part of cry4Ba and the C-terminus part of cry1Ac, was constructed. Its transformation to an acrystalliferous B. thuringiensis strain showed that it was expressed as a chimerical protein of 116 kDa, assembled in spherical to amorphous parasporal crystals. The chimerical gene cry(4Ba-1Ac) was introduced in a B. thuringiensis kurstaki strain. In the generated crystals of the recombinant strain, the presence of Cry(4Ba-1Ac) was evidenced by MALDI-TOF. The recombinant strain showed an important increase of the toxicity against Culex pipiens larvae (LC50 = 0.84 mg l(-1) +/- 0.08) compared to the wild type strain through the synergistic activity of Cry2Aa with Cry(4Ba-1Ac). The enhancement of toxicity of B. thuringiensis kurstaki expressing Cry(4Ba-1Ac) compared to that expressing the native toxin Cry4Ba, might be related to its a typical crystallization properties. The developed fusion protein could serve as a potent toxin against different pests of mosquitoes and major crop plants.
%$ 084 ; 020 ; 052