@article{fdi:010068850,
  title = {{M}odification of a commercial {DNA} extraction kit for safe and rapid recovery of {DNA} and {RNA} simultaneously from soil, without the use of harmful solvents},
  author = {{T}ournier, {E}stelle and {A}menc, {L}. and {P}ablo, {A}nne-{L}aure and {L}egname, {E}. and {B}lanchart, {E}ric and {P}lassard, {C}. and {R}obin, {A}. and {B}ernard, {L}aetitia},
  editor = {},
  language = {{ENG}},
  abstract = {{A}n optimized method, based on the coupling of two commercial kits, is described for the extraction of soil nucleic acids, with simultaneous extraction and purification of {DNA} and {RNA} following a cascade scheme and avoiding the use of harmful solvents. {T}he protocol canmonitor the variations in the recovery yield of {DNA} and {RNA} from soils of various types. {T}he quantitative version of the protocol was obtained by testing the starting soil quantity, the grinding parameters and the final elution volumes, in order to avoid saturation of both kits. {A} first soil-crushing step in liquid nitrogen could be added for the assessment of fungal parameters. {T}he protocol was efficient on different tropical soils, including {A}ndosol, while their high contents of clays, including poorly crystalline clays, and {F}e and {A}l oxides usually make the nucleic acid extraction more difficult. {T}he {RNA} recovery yield from the previous tropical soils appeared to correlate better to soil respiration than {DNA}, which is positively influenced by soil clay content.},
  keywords = {{S}oil {DNA} and {RNA} ; {Q}uantitative co-extraction ; {M}olecular biomass ; {T}ropical soil ; {MADAGASCAR} ; {ZONE} {TROPICALE}},
  booktitle = {},
  journal = {{M}ethods{X}},
  volume = {2},
  numero = {},
  pages = {182--191},
  ISSN = {2215-0161},
  year = {2015},
  DOI = {10.1016/j.mex.2015.03.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068850},
}