Publications des scientifiques de l'IRD

Bangoura N., Diouara A. A. M., Cisse M., Ndiaye H. D., Mboup S., Ayouba Ahidjo, Kane C. T. (2015). Quantification of viral load and resistance tests of HIV-1 to ARVs from dried blood spots samples in Guinean patients undergoing antiretroviral treatment. African Journal of Laboratory Medicine, 4 (1), p. art. 168 [7 p.]. ISSN 2225-2002.

Titre du document
Quantification of viral load and resistance tests of HIV-1 to ARVs from dried blood spots samples in Guinean patients undergoing antiretroviral treatment
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000360140400002
Auteurs
Bangoura N., Diouara A. A. M., Cisse M., Ndiaye H. D., Mboup S., Ayouba Ahidjo, Kane C. T.
Source
African Journal of Laboratory Medicine, 2015, 4 (1), p. art. 168 [7 p.] ISSN 2225-2002
Problem: As in several countries of the South, the virological monitoring of patients undergoing antiretroviral treatment (ARVT) in Guinea is low or non-existent in some locations. The aim of this study was to assess the technical and logistical feasibility of the use of (dried blood spots) DBSs in viral load (VL) and genotyping tests. Method: From September 2010 to October 2010, DBS were prepared from blood samples of adult patients under ARVT. The samples had to be sent to the reference laboratory within 30 days after the sample had been done at ambient temperature. The VL was quantified and the samples of patients with virological failure (CV >= 3 log(10) copies/mL) were genotyped according to the ANRS protocol. The Stanford algorithm, version 6.0.8, was used to analyse and interpret the resistance mutations. Results: Amongst the 136 included patients, 129 and 7 were under first and second line treatment respectively, and monitored for an average of 35 months [IQR: 6-108]. Virological failure was noticed among 33 patients. Among them, 84.8% (n = 28/33) benefited from genotyping. The global resistance rate was 14% (n = 19/136). CRF02_AG was the most prevalent viral subtype (82%; n = 23) Conclusion: In addition to demonstrating the technical and logistic feasibility of VL and genotyping tests from DBSs, these results show the relevance of their use in the virological monitoring of patients under ARVT. Also, this study made it possible to provide information on virological failure, ARV resistance and the HIV-1 genetic diversity in Guinea.
Plan de classement
Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
GUINEE
Localisation
Fonds IRD [F B010068837]
Identifiant IRD
fdi:010068837
Contact