Breitler J. C. auteur aut IRD Campa Claudine auteur aut IRD Georget F. auteur aut IRD Bertrand B. auteur aut IRD Etienne H. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical plants in the field. RNA extraction directly in a complex field environment opens up the way for studying gene-environment interactions at transcriptome level to decipher the complex regulatory network involved in multiple-stress responses. specialized ZONE TROPICALE 076 020 6 art. 38368 [6 p.] 2016 2045-2322 https://www.documentation.ird.fr/hor/fdi:010068775 10.1038/srep38368 2045-2322 [F B010068775] https://www.documentation.ird.fr/hor/fdi:010068775 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-01/010068775.pdf IRD - Base Horizon / Pleins textes 2017-02-06 2017-08-23 fdi:010068775 fre