@article{fdi:010068359,
  title = {{N}if{DK} clusters located on the chromosome and megaplasmid of {B}radyrhizobium sp strain {DOA}9 contribute differently to nitrogenase activity during symbiosis and free-living growth},
  author = {{W}ongdee, {J}. and {S}ongwattana, {P}. and {N}ouwen, {N}ico and {N}oisangiam, {R}. and {F}ardoux, {J}o{\¨e}l and {C}haintreuil, {C}l{\'e}mence and {T}eaumroong, {N}. and {T}ittabutr, {P}. and {G}iraud, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{B}radyrhizobium sp. strain {DOA}9 contains two copies of the nif{DK} genes, nif{DK}c, located on the chromosome, and nif{DK}p, located on a symbiotic megaplasmid. {U}nlike most rhizobia, this bacterium displays nitrogenase activity under both free-living and symbiotic conditions. {T}ranscriptional analysis using gus{A} reporter strains showed that both nif{DK} operons were highly expressed under symbiosis, whereas nif{DK}c was the most abundantly expressed under free-living conditions. {D}uring free-living growth, the nif{DK}p mutation did not affect nitrogenase activity, whereas nitrogenase activity was drastically reduced with the nif{DK}c mutant. {T}his led us to suppose that nif{DK}c is the main contributor of nitrogenase activity in the free-living state. {I}n contrast, during symbiosis, no effect of the nif{DK}c mutation was observed and the nitrogen-fixation efficiency of plants inoculated with the nif{DK}p mutant was reduced. {T}his suggests that nif{DK}p plays the main role in nitrogenase enzyme activity during symbiosis. {T}ogether, these data suggest that {B}radyrhizobium sp. strain {DOA}9 contains two functional copies of nif{DK} genes that are regulated differently and that, depending on their lifestyle, contribute differently to nitrogenase activity.},
  keywords = {},
  booktitle = {},
  journal = {{M}olecular {P}lant-{M}icrobe {I}nteractions},
  volume = {29},
  numero = {10},
  pages = {767--773},
  ISSN = {0894-0282},
  year = {2016},
  DOI = {10.1094/mpmi-07-16-0140-r},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068359},
}