Borsa Philippe auteur aut IRD Shen K. N. auteur aut IRD Arlyza I. S. auteur aut IRD Hoareau T. B. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Previous investigations have uncovered divergent mitochondrial clades within the blue-spotted maskray, previously Neotrygon kuhlii (Muller and Henle). The hypothesis that the blue-spotted maskray may consist of a complex of multiple cryptic species has been proposed, and four species have been recently described or resurrected. To test the multiple cryptic species hypothesis, we investigated the phylogenetic relationships and coalescence patterns of mitochondrial sequences in a sample of 127 new individuals from the Indian Ocean and the Coral Triangle region, sequenced at both the CO1 and cytochrome b loci. The maximum-likelihood (ML) tree of concatenated CO1 + cytochrome b gene sequences, rooted by the New Caledonian maskray N. trigonoides, yielded 9 strongly supported, main clades. Puillandre's ABGD algorithm detected gaps in nucleotide distance consistent with the ML phylogeny. The general mixed Yule-coalescent algorithm partitioned the dataset into putative species generally consistent with the ML phylogeny. Nuclear markers generally confirmed that distinct mitochondrial clades correspond to genetically isolated lineages. The nine main lineages identified by ML analysis were geographically distributed in a parapatric fashion, indicating reproductive isolation. The hypothesis of multiple cryptic species is thus validated. specialized Parapatric distribution Neotrygon kuhlii Neotrygon trigonoides Indo-West Pacific Coral Triangle PACIFIQUE OCEAN INDIEN NOUVELLE CALEDONIE INDONESIE PHILIPPINES MALAISIE AFRIQUE DE L'EST 034 339 9-10 417-426 2016 1631-0691 https://www.documentation.ird.fr/hor/fdi:010068328 10.1016/j.crvi.2016.07.004 1631-0691 [F B010068328] https://www.documentation.ird.fr/hor/fdi:010068328 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-05/010068328.pdf IRD - Base Horizon / Pleins textes 2017-01-09 2017-08-23 fdi:010068328 fre