@article{fdi:010068318,
  title = {{T}oxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of {B}acillus thuringiensis crystal protein {C}ry2{A}a},
  author = {{E}lleuch, {J}. and {J}aoua, {S}. and {G}inibre, {C}arole and {C}handre, {F}abrice and {T}ounsi, {S}. and {Z}ghal, {R}. {Z}.},
  editor = {},
  language = {{ENG}},
  abstract = {{BACKGROUNDB}acillus thuringiensis -endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. {I}mproving their efficacy is of great benefit. {H}ere, an improvement in {C}ry2{A}a-endotoxin toxicity was attempted via a cry gene over expression system using {P}20 from {B}. thuringiensis israelensis. {RESULTST}he coexpression of {C}ry2{A}a with {P}20 resulted in a seven fold increase in its production yield in {B}. thuringiensis. {G}enerated crystals proved to be significantly more toxic (505.207 mu gg(-1), 1.99mg{L}(-1) and 1.49mg{L}(-1)) than the {P}20-lacking control (720.78 mu gg(-1), 705.69mg{L}(-1) and 508.51mg{L}(-1)) against {E}phestia kuehniella, {A}edes aegypti and {C}ulex pipiens larvae respectively. {I}n vitro, processing experiments revealed a {P}20-mediated protection of {C}ry2{A}a against degradation under larval gut conditions. {T}hus, {P}20 could promote the maintenance of a tightly packaged conformation of {C}ry2{A}a toxins in the larval midgut upon correct activation and binding to its membrane receptors. {CONCLUSIONB}ased on their resistance against excessive proteolysis, {C}ry2{A}a-endotoxins, produced in the presence of {P}20, could be considered as a successful control agent for {E}. kuehniella and an effective alternative for mosquito control, implying its possible exploitation in pest management programmes.},
  keywords = {{P}20 helper protein ; {C}ry2{A}a toxin ; larvicidal activity improvement ; protection against excessive proteolysis},
  booktitle = {},
  journal = {{P}est {M}anagement {S}cience},
  volume = {72},
  numero = {12},
  pages = {2240--2246},
  ISSN = {1526-498{X}},
  year = {2016},
  DOI = {10.1002/ps.4261},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068318},
}