@article{fdi:010068288, title = {{F}luorescence in situ hybridization of {M}icrocystis strains producing microcystin using specific m{RNA} probes}, author = {{Z}eller, {P}. and {M}ejean, {A}. and {B}iegala, {I}sabelle and {C}ontremoulins, {V}. and {P}loux, {O}.}, editor = {}, language = {{ENG}}, abstract = {{C}yanobacteria are ubiquitous micro-organisms that can produce toxic compounds, the cyanotoxins. {T}he monitoring of such producers in the environment is of prime importance for human health. {A}n attractive technology for such monitoring is fluorescence insitu hybridization ({FISH}), which allows the detection and enumeration of environmental micro-organisms. {W}e present here the application of tyramide signal amplification fluorescence insitu hybridization ({TSA}-{FISH}) to the detection of microcystin-producing {M}icrocystis strains. {W}e used a 16{S} r{RNA}-specific probe, {MICR}3, to specifically label and observe by epifluorescence microscopy {M}icrocystis aeruginosa strains. {U}sing confocal laser scanning microscopy and a specific probe, {MCYA}, targeting the mcy{A} m{RNA} we have labelled {M}.aeruginosa {PCC} 7806, which produces microcystins. {M}icrocystis aeruginosa {PCC} 7005 which does not produce microcystins is not labelled by this probe. {F}urthermore, we show here that this specific m{RNA} labelling in {M}.aeruginosa {PCC} 7806 is enhanced in cells illuminated for 1h just after a dark period of cultivation of 24h, conditions in which the mcy{A} gene is up regulated. {T}he data presented here might be applicable to the monitoring of toxic {M}icrocystis strains in the environment. {S}ignificance and {I}mpact of the {S}tudy{C}yanobacteria producing toxic compounds (cyanotoxins) are present in the environment and in water bodies. {T}heir presence poses a threat on human and animal health. {I}t is thus important to detect, identify and enumerate these toxic {C}yanobacteria. {U}sing tyramide signal amplification fluorescence insitu hybridization ({TSA}-{FISH}) and specific probes, with confocal laser scanning microscopy, we have specifically detected {M}icrocystis strains producing microcystin toxins. {T}he data presented here might be applied to the monitoring of water bodies at early stages and all along the formation of {M}icrocystis blooms.}, keywords = {{C}yanobacteria ; mcy{A} ; microcystin ; {M}icrocystis ; tyramide signal amplification fluorescence insitu hybridization}, booktitle = {}, journal = {{L}etters in {A}pplied {M}icrobiology}, volume = {63}, numero = {5}, pages = {376--383}, ISSN = {0266-8254}, year = {2016}, DOI = {10.1111/lam.12634}, URL = {https://www.documentation.ird.fr/hor/fdi:010068288}, }