%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Guichet, Emilande
%A Aghokeng Fobang, Avelin
%A Eymard-Duvernay, Sabrina
%A Vidal, Nicole
%A Ayouba, Ahidjo
%A Ngole, E. M.
%A Delaporte, Eric
%A Ciaffi, L.
%A Peeters, Martine
%T Field evaluation of an open and polyvalent universal HIV-1/SIVcpz/SIVgor quantitative RT-PCR assay for HIV-1 viral load monitoring in comparison to Abbott RealTime HIV-1 in Cameroon
%D 2016
%L fdi:010068286
%G ENG
%J Journal of Virological Methods
%@ 0166-0934
%K HIV-1 ; Viral load ; Genetic diversity ; Subtype ; Africa
%K CAMEROUN
%M ISI:000386190700019
%P 121-126
%R 10.1016/j.jviromet.2016.09.005
%U https://www.documentation.ird.fr/hor/fdi:010068286
%> https://www.documentation.ird.fr/intranet/publi/2016/11/010068286.pdf
%V 237
%W Horizon (IRD)
%X With the increasing demand of HIV viral load (VL) tests in resource-limited countries (RLCs) there is a need for assays at affordable cost and able to quantify all known HIV-1 variants. VLs obtained with a recently developed open and polyvalent universal HIV-1 /SIVcpz/SIVgor RT-qPCR were compared to Abbott RealTime HIV-1 assay in Cameroon. On 474 plasma samples, characterized by a wide range of VLs and a broad HIV-1 group M genetic diversity, 97.5% concordance was observed when using the lower detection limit of each assay. When using the threshold of 3.00 log(10) copies/mL, according to WHO guidelines to define virological failure (VF) in RLCs, the concordance was 94.7%, 360/474 versus 339/474 patients were identified with VF with the new assay and Abbott RealTime HIV-1, respectively. Higher VLs were measured with the new assay, +0.47 log(10) copies/mL (95% CI; 0.42-0.52) as shown with Bland-Altman analysis. Eleven samples from patients on VF with drug resistance were not detected by Abbott RealTime HIV-1 versus two only with the new assay. Overall, our study showed that the new assay can be easily implemented in a laboratory in RLCs with VL experience and showed good performance on a wide diversity of HIV-1 group M variants.
%$ 052