@article{fdi:010068286,
  title = {{F}ield evaluation of an open and polyvalent universal {HIV}-1/{SIV}cpz/{SIV}gor quantitative {RT}-{PCR} assay for {HIV}-1 viral load monitoring in comparison to {A}bbott {R}eal{T}ime {HIV}-1 in {C}ameroon},
  author = {{G}uichet, {E}milande and {A}ghokeng {F}obang, {A}velin and {E}ymard-{D}uvernay, {S}abrina and {V}idal, {N}icole and {A}youba, {A}hidjo and {N}gole, {E}. {M}. and {D}elaporte, {E}ric and {C}iaffi, {L}. and {P}eeters, {M}artine},
  editor = {},
  language = {{ENG}},
  abstract = {{W}ith the increasing demand of {HIV} viral load ({VL}) tests in resource-limited countries ({RLC}s) there is a need for assays at affordable cost and able to quantify all known {HIV}-1 variants. {VL}s obtained with a recently developed open and polyvalent universal {HIV}-1 /{SIV}cpz/{SIV}gor {RT}-q{PCR} were compared to {A}bbott {R}eal{T}ime {HIV}-1 assay in {C}ameroon. {O}n 474 plasma samples, characterized by a wide range of {VL}s and a broad {HIV}-1 group {M} genetic diversity, 97.5% concordance was observed when using the lower detection limit of each assay. {W}hen using the threshold of 3.00 log(10) copies/m{L}, according to {WHO} guidelines to define virological failure ({VF}) in {RLC}s, the concordance was 94.7%, 360/474 versus 339/474 patients were identified with {VF} with the new assay and {A}bbott {R}eal{T}ime {HIV}-1, respectively. {H}igher {VL}s were measured with the new assay, +0.47 log(10) copies/m{L} (95% {CI}; 0.42-0.52) as shown with {B}land-{A}ltman analysis. {E}leven samples from patients on {VF} with drug resistance were not detected by {A}bbott {R}eal{T}ime {HIV}-1 versus two only with the new assay. {O}verall, our study showed that the new assay can be easily implemented in a laboratory in {RLC}s with {VL} experience and showed good performance on a wide diversity of {HIV}-1 group {M} variants.},
  keywords = {{HIV}-1 ; {V}iral load ; {G}enetic diversity ; {S}ubtype ; {A}frica ; {CAMEROUN}},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {237},
  numero = {},
  pages = {121--126},
  ISSN = {0166-0934},
  year = {2016},
  DOI = {10.1016/j.jviromet.2016.09.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068286},
}