Sbabou L. auteur aut IRD Regragui A. auteur aut IRD Filali-Maltouf A. auteur aut IRD Ater M. auteur aut IRD Béna Gilles auteur aut IRD text journalArticle eng text/pdf born digital access A total of 212 symbiotic bacteria were isolated from nodules of Vicia ervilia, a traditional crop cultivated in Northern Morocco. The isolates were recovered from 10 different sites, trapped each time with the local cultivar grown in the same field. Four loci were sequenced in order to characterize the isolates, including two housekeeping genes (recA and glnII), one plasmidic symbiotic gene (nodC) and one locus from another plasmid (prL11). In several isolates, two different copies of glnII were detected and sequenced, suggesting a unique duplication event, which has never been reported previously. There was no correlation between the genetic differentiation among cultivars and among bacteria, showing that the evolution of the bacterial population was independent, at least partially, from the host plant. By placing the haplo-types-in a wide-ranging phylogenetic reconstruction, it was shown that the diversity detected in Morocco was spread throughout the different clades detected worldwide. The differentiation between areas relied on frequency variations of haplotypes rather than a presence/absence pattern. This finding raises new questions concerning bacterial genetic resource preservation, and confirms the old tenet "everything is everywhere but the environment selects" specialized Population genetics Symbiosis Legume Recombination Local adaptation Rhizobium MAROC 084 39 6 409-417 2016 0723-2020 https://www.documentation.ird.fr/hor/fdi:010068144 10.1016/j.syapm.2016.06.005 0723-2020 [F B010068144] https://www.documentation.ird.fr/hor/fdi:010068144 https://www.documentation.ird.fr/intranet/publi/2016/10/010068144.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2016-11-03 2017-08-23 fdi:010068144 fre