@article{fdi:010067637,
  title = {{P}inpointing the level of isolation between two cryptic species sharing the same microhabitat : a case study with a scarabaeid species complex},
  author = {{R}oy, {L}. and {B}on, {M}. {C}. and {C}esarini, {C}. and {S}erin, {J}. and {B}onato, {O}livier},
  editor = {},
  language = {{ENG}},
  abstract = {{U}nderstanding the processes of speciation is an important challenge in improving knowledge of the origin of biodiversity. {O}ne crucial point is to assess the causes of reproductive isolation, especially in the case of co-occurring species. {D}ifferences in microscale spatial distribution in small organisms may blur the issue. {W}e explored biological processes underlying speciation within dung beetles belonging to the vacca species complex ({S}carabaeidae: {O}nthophagus). {T}he two taxa of this complex, {O}.vacca and {O}.medius, not only are known to have a large overlapping {P}alearctic distribution range but also share the same cowpat with no physical barriers and no observed specific aggregated patterns in the local distribution. {T}he present study aimed at determining the level of isolation between the two taxa and discusses the most likely scenario of the speciation (sympatry vs. allopatry) based on the {C}oyne & {O}rr's (2004) four criteria. {W}e conducted a full study on populations sampled within the {M}editerranean region integrating morphological analysis (digital image analysis of the elytral melanism pattern), two-gene phylogenies, population genetic analyses on populations sampled from an area where both species occur and another one with {O}.vacca only, as well as intra- and interspecific mating and crossing bioassays. {T}he variation in the elytral melanism pattern clearly followed a bimodal distribution, with {O}.medius being more melanic than {O}.vacca, with a very limited overlapping area. {T}he two taxa are reproductively isolated, with a strong postzygotic incompatibility despite the absence of sexual isolation. {S}equence analysis of both nuclear and mitochondrial markers revealed a deep divergence between the two taxa dating back to 8.7{M}ya. {A}ll findings concurred with some phenological observations and the conclusion that the most likely scenario for speciation in the vacca complex was an allopatric speciation followed by secondary contact.},
  keywords = {{FRANCE} ; {MAROC}},
  booktitle = {},
  journal = {{Z}oologica {S}cripta},
  volume = {45},
  numero = {4},
  pages = {407--420},
  ISSN = {0300-3256},
  year = {2016},
  DOI = {10.1111/zsc.12166},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067637},
}