Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Journal of Virological Methods 51-55 HBV drug resistance Oligonucleotide ligation assay Lamivudine resistance HBV treatment THAILANDE 2016 fdi:010066848 ENG Journal of Virological Methods 0166-0934 ISI:000375506400009 10.1016/j.jviromet.2016.03.014 https://www.documentation.ird.fr/hor/fdi:010066848 https://www.documentation.ird.fr/intranet/publi/2016/05/010066848.pdf 233 Horizon (IRD) Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings. 052 ; 050 UR174 Thaïlande