Castelli E. C. auteur aut IRD Mendes C. T. auteur aut IRD Sabbagh A. auteur aut IRD Porto I. O. P. auteur aut IRD Garcia André auteur aut IRD Ramalho J. auteur aut IRD Lima T. H. A. auteur aut IRD Massaro J. D. auteur aut IRD Dias F. C. auteur aut IRD Collares C. V. A. auteur aut IRD Jamonneau Vincent auteur aut IRD Bucheton Bruno auteur aut IRD Camara M. auteur aut IRD Donadi E. A. auteur aut IRD text journalArticle eng text/pdf born digital access HLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes. specialized West-African populations HLA-E Next-generation sequencing (NGS) Polymorphisms Haplotypes Non-classical HLA Africa GUINEE BURKINA FASO 050 106 76 12 945-953 2015 0198-8859 https://www.documentation.ird.fr/hor/fdi:010066010 10.1016/j.humimm.2015.06.016 0198-8859 [F B010066010] https://www.documentation.ird.fr/hor/fdi:010066010 https://www.documentation.ird.fr/intranet/publi/2016/01/010066010.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2016-02-02 2017-08-23 fdi:010066010 fre