%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Castelli, E. C.
%A Mendes, C. T.
%A Sabbagh, A.
%A Porto, I. O. P.
%A Garcia, André
%A Ramalho, J.
%A Lima, T. H. A.
%A Massaro, J. D.
%A Dias, F. C.
%A Collares, C. V. A.
%A Jamonneau, Vincent
%A Bucheton, Bruno
%A Camara, M.
%A Donadi, E. A.
%T HLA-E coding and 3 ' untranslated region variability determined by next-generation sequencing in two West-African population samples
%D 2015
%L fdi:010066010
%G ENG
%J Human Immunology
%@ 0198-8859
%K West-African populations ; HLA-E ; Next-generation sequencing (NGS) ; Polymorphisms ; Haplotypes ; Non-classical HLA ; Africa
%K GUINEE ; BURKINA FASO
%M ISI:000366437900011
%N 12
%P 945-953
%R 10.1016/j.humimm.2015.06.016
%U https://www.documentation.ird.fr/hor/fdi:010066010
%> https://www.documentation.ird.fr/intranet/publi/2016/01/010066010.pdf
%V 76
%W Horizon (IRD)
%X HLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes.
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