@article{fdi:010066010,
  title = {{HLA}-{E} coding and 3 ' untranslated region variability determined by next-generation sequencing in two {W}est-{A}frican population samples},
  author = {{C}astelli, {E}. {C}. and {M}endes, {C}. {T}. and {S}abbagh, {A}. and {P}orto, {I}. {O}. {P}. and {G}arcia, {A}ndr{\'e} and {R}amalho, {J}. and {L}ima, {T}. {H}. {A}. and {M}assaro, {J}. {D}. and {D}ias, {F}. {C}. and {C}ollares, {C}. {V}. {A}. and {J}amonneau, {V}incent and {B}ucheton, {B}runo and {C}amara, {M}. and {D}onadi, {E}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{HLA}-{E} is a non-classical {H}uman {L}eucocyte {A}ntigen class {I} gene with immunomodulatory properties. {W}hereas {HLA}-{E} expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with {NK} cells and {T} lymphocytes, being important for immunosurveillance and also for fighting against infections. {HLA}-{E} is usually the most conserved locus among all class {I} genes. {H}owever, most of the previous studies evaluating {HLA}-{E} variability sequenced only a few exons or genotyped known polymorphisms. {H}ere we report a strategy to evaluate {HLA}-{E} variability by next-generation sequencing ({NGS}) that might be used to other {HLA} loci and present the {HLA}-{E} haplotype diversity considering the segment encoding the entire {HLA}-{E} m{RNA} (including 5'{UTR}, introns and the 3'{UTR}) in two {A}frican population samples, {S}usu from {G}uinea-{C}onakry and {L}obi from {B}urkina {F}aso. {O}ur results indicate that (a) the {HLA}-{E} gene is indeed conserved, encoding mainly two different protein molecules; (b) {A}fricans do present several unknown {HLA}-{E} alleles presenting synonymous mutations; (c) the {HLA}-{E} 3'{UTR} is quite polymorphic and (d) haplotypes in the {HLA}-{E} 3'{UTR} are in close association with {HLA}-{E} coding alleles. {NGS} has proved to be an important tool on data generation for future studies evaluating variability in non-classical {MHC} genes.},
  keywords = {{W}est-{A}frican populations ; {HLA}-{E} ; {N}ext-generation sequencing ({NGS}) ; {P}olymorphisms ; {H}aplotypes ; {N}on-classical {HLA} ; {A}frica ; {GUINEE} ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{H}uman {I}mmunology},
  volume = {76},
  numero = {12},
  pages = {945--953},
  ISSN = {0198-8859},
  year = {2015},
  DOI = {10.1016/j.humimm.2015.06.016},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066010},
}