@article{fdi:010065794,
  title = {{N}on-host resistance responses of {A}rabidopsis thaliana to the coffee leaf rust fungus ({H}emileia vastatrix)},
  author = {{A}zinheira, {H}. {G}. and {S}ilva, {M}. {D}. and {T}alhinhas, {P}. and {M}edeira, {C}. and {M}aia, {I}. and {P}etitot, {A}nne-{S}ophie and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{L}eaf rust, caused by {H}emileia vastatrix {B}erk & {B}roome, is the most destructive fungal disease of coffee. {I}n the absence of a suitable gene validation system in coffee, the objective of this study was to investigate whether the model plant {A}rabidopsis thaliana ({L}.) {H}eynh. may be used as a heterologous system for the molecular dissection of coffee responses to leaf rust. {H}istological examination of {A}. thaliana ({C}ol-0) leaves inoculated with {H}. vastatrix (race {II}) showed that by 24 h after inoculation (hai), {H}. vastatrix uredospores differentiated appressoria and penetrated the stomata, but failed to form haustoria. {A}rabidopsis thaliana cellular resistance responses included hypersensitive-like response ({HR}) of stomata guard cells together with accumulation of phenolic compounds and callose deposition in walls of epidermal and mesophyll cells. {R}esults indicate that {H}. vastatrix infection triggered the induction of a set of defence-related genes peaking at 18 and 42 hai. {T}he non-host {HR} triggered by {H}. vastatrix in the model plant {A}. thaliana makes it usable to infer the function of coffee genes involved in pre-haustorial rust resistance. {L}a rouille orang{\'e}e du caf{\'e}ier, provoqu{\'e}e par {H}emileia vastatrix {B}erk & {B}roome, est la maladie fongique la plus dommageable {\`a} la culture du caf{\'e}. {E}n l'absence d'un syst{\`e}me appropri{\'e} de validation fonctionnelle de g{\`e}nes chez le caf{\'e}ier, l'objectif de cette {\'e}tude {\'e}tait d'{\'e}tudier si la plante mod{\`e}le {A}rabidopsis thaliana ({L}.) {H}eynh. pouvait {\^e}tre employ{\'e}e comme syst{\`e}me h{\'e}t{\'e}rologue pour la dissection mol{\'e}culaire des r{\'e}ponses du caf{\'e}ier {\`a} la rouille orang{\'e}e. {L}'examen histologique des feuilles d'{A}.thaliana ({C}ol-0) inocul{\'e}es avec {H}.vastatrix (race {II}) a montr{\'e} que 24 heures apr{\`e}s inoculation (hai), les ur{\'e}dospores d'{H}.vastatrix avaient diff{\'e}renci{\'e} des appressoria et p{\'e}n{\'e}tr{\'e} par les stomates, mais n'avaient pas r{\'e}ussi {\`a} former des haustoria. {A}u plan cellulaire, les r{\'e}ponses de r{\'e}sistance d'{A}.thaliana se traduisent par une r{\'e}action d'hypersensibilit{\'e} ({HR}) des cellules de garde des stomates, ainsi que par l'accumulation de compos{\'e}s ph{\'e}noliques et le d{\'e}p{\^o}t de callose dans les parois des cellules {\'e}pidermiques et du m{\'e}sophylle. {A}u plan mol{\'e}culaire, les {\'e}tudes d'expression des g{\`e}nes indiquent que l'infection par {H}.vastatrix d{\'e}clenche l'activation d'un ensemble de g{\`e}nes li{\'e}s {\`a} la d{\'e}fense et la signalisation de la r{\'e}sistance, avec un pic d'induction vers 18 hai et 42 hai. {C}es r{\'e}sultats indiquent que les r{\'e}ponses de r{\'e}sistance non-h{\^o}te d{\'e}clench{\'e}es par {H}.vastatrix chez la plante mod{\`e}le {A}.thaliana sont utilisables pour {\'e}valuer la fonction des g{\`e}nes de caf{\'e}ier impliqu{\'e}s dans la r{\'e}sistance pr{\'e}-haustoriale {\`a} la rouille.},
  keywords = {{C}offee arabica ; hypersensitive response ; defence gene expression ; {PR} ; genes},
  booktitle = {},
  journal = {{B}otany - {B}otanique},
  volume = {88},
  numero = {7},
  pages = {621--629},
  ISSN = {1916-2790},
  year = {2010},
  DOI = {10.1139/b10-039},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065794},
}