@article{fdi:010065790,
  title = {{C}offee ({C}offea arabica {L}.) genes early expressed during infection by the rust fungus ({H}emileia vastatrix)},
  author = {{F}ernandez, {D}. and {S}antos, {P}. and {A}gostini, {C}. and {B}on, {M}. {C}. and {P}etitot, {A}. {S}. and {S}ilva, {M}. {C}. and {G}uerra {G}uimaraes, {L}. and {R}ibeiro, {A}. and {A}rgout, {X}. and {N}icole, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he beverage cash crop coffee ({C}offea arabica {L}.) is subject to severe losses caused by the rust fungus {H}emileia vastatrix. {I}n naturally resistant coffee plants, a specific hypersensitive reaction ({HR}) may be elicited early to stop fungal infection. {T}o isolate host genes involved in {HR}, we undertook an expressed sequence tags ({EST}s) analysis. {T}wo c{DNA} libraries were constructed using suppression subtractive hybridization ({SSH}) and 527 non-redundant {EST}s were generated from 784 randomly picked clones. {C}lassification of the {EST}s into several functional categories showed that more than one-quarter of the predicted proteins might encode disease resistance ({R}) proteins, stress- and defence-proteins, and components of signal transduction pathways. {T}wenty-eight differentially screened sequences ({DSS}s) were selected after differential hybridization of 1000 c{DNA} clones from each library. {I}nvestigation of the expression patterns of a subset of 13 {DSS}s showed higher levels of gene expression in inoculated plants compared with control plants. {HR}-up-regulation of transcript accumulation occurred for 9 out of the 13 genes 24 and 48 h after {H}. vastatrix challenge. {T}wo genes encoded homologues of the {A}rabidopsis {DND}1 and {NDR}1 proteins, suggesting conservation of resistance signalling pathways in perennial plants. {O}ther {HR}-regulated sequences matched receptor kinases, {AP}2 domain- and {WRKY} transcription factors, cytochromes {P}450, heat shock 70 proteins, glucosyltransferases and proteins of unknown function. {T}he {EST}s reported here provide a useful resource for studying coffee resistance responses and for improving {C} arabica for durable disease resistance.},
  keywords = {},
  booktitle = {},
  journal = {{M}olecular {P}lant {P}athology},
  volume = {5},
  numero = {6},
  pages = {527--536},
  ISSN = {1464-6722},
  year = {2004},
  DOI = {10.1111/j.1364-3703.2004.00250.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065790},
}