Fadhlaoui K. auteur aut IRD Ben Hania Wajdi auteur aut IRD Postec A. auteur aut IRD Fauque G. auteur aut IRD Hamdi M. auteur aut IRD Ollivier Bernard auteur aut IRD Fardeau Marie-Laure auteur aut IRD text journalArticle eng text/pdf born digital access Strain KhalAKB1(T), a mesophilic, anaerobic, rod-shaped bacterium, was isolated from water collected from a mesothermic Tunisian spring. Cells were Gram-staining-Positive rods, occurring singly or in pairs and motile by one lateral flagellum. Strain KhalAKB1(T) grew at 15-45 degrees C (optimum 30 degrees C), at pH 5.5-8.5 (optimum pH 7.0) and in the presence of 0-35 g NaCl l(-1) (optimum 1 g NaCl l(-1)). It fermented yeast extract and a wide range of carbohydrates including cellobiose, D-glucose, D-ribose, sucrose, D-xylose, maltose, D-galactose and starch as electron donors. Acetate, ethanol, CO2 and H-2 were end products of glucose metabolism. It reduced elemental sulfur, but not sulfate, thiosulfate or sulfite, into sulfide. The DNA G+C content was 37.6 mol%. The predominant cellular fatty acids were C-14 : 0 and C-16 : 0. Phylogenetic analysis of the 16S rRNA gene sequence suggested Fusibacter bizertensis as the closest relative of this isolate (identity of 97.2 % to the type strain). Based on phenotypic, phylogenetic and genotypic taxonomic characteristics, strain KhalAKB1(T) is proposed to be assigned to a novel species within the genus Fusibacter, order Clostridiales, Fusibacter fontis sp. nov. The type strain is KhalAKB1(T) (=DSM 28450(T)=JCM 19912(T)). specialized TUNISIE BIZERTE 084 65 10 3501-3506 2015 1466-5026 https://www.documentation.ird.fr/hor/fdi:010065515 10.1099/ijsem.0.000445 1466-5026 [F B010065515] https://www.documentation.ird.fr/hor/fdi:010065515 https://www.documentation.ird.fr/intranet/publi/2015/12/010065515.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2016-01-14 2024-09-11 fdi:010065515 fre