@article{fdi:010065388,
  title = {{A}ntibodies to {P}lasmodium falciparum merozoite surface protein-1p19 malaria vaccine candidate induce antibody-dependent respiratory burst in human neutrophils},
  author = {{J}oos, {C}. and {V}arela, {M}. {L}. and {M}bengue, {B}. and {M}ansourou, {A}. and {M}arrama, {L}. and {S}okhna, {C}heikh and {T}all, {A}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {T}oure, {A}. and {M}ercereau-{P}uijalon, {O}. and {P}erraut, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {I}dentification of plasmodial antigens targeted by protective immune mechanisms is important for malaria vaccine development. {A}mong functional assays, the neutrophil antibody-dependent respiratory burst ({ADRB}) induced by opsonized {P}lasmodium falciparum merozoites has been correlated with acquired immunity to clinical malaria in endemic areas, but the target merozoite antigens are unknown. {H}ere, the contribution of antibodies to the conserved {C}-terminal domain of the {P}. falciparum merozoite surface protein-1 ({P}f{MSP}1p19) in mediating {ADRB} was investigated in sera from individuals living in two {S}enegalese villages with differing malaria endemicity. {M}ethods: {A}nti-{P}f{MSP}1p19 antibody levels in sera from 233 villagers were investigated and the involvement of anti-{P}f{MSP}1p19 antibodies in {ADRB} was explored in a subset of samples using (1) isogenic {P}. falciparum parasite clones expressing {P}. falciparum or {P}lasmodium chabaudi {MSP}1p19; (2) {P}f{MSP}1p19-coated plaque {ADRB}; and, (3) {ADRB} triggering using sera depleted from {P}f{MSP}1p19 antibodies by absorption onto the baculovirus recombinant antigen. {R}esults: {ADRB} activity correlated with anti-{P}f{MSP}1p19 {I}g{G} levels ({P} < 10(-3)). {A} substantial contribution of {P}f{MSP}1p19 antibody responses to {ADRB} was confirmed ({P} < 10(-4)) in an age-adjusted linear regression model. {P}f{MSP}1p19 antibodies accounted for 33.1 % (range 7-54 %) and 33.2 % (range 0-70 %) of {ADRB} activity evaluated using isogenic merozoites ({P} < 10(-3)) and depleted sera ({P} = 0.0017), respectively. {C}oating of {P}f{MSP}1p19 on plates induced strong {ADRB} in anti-{P}f{MSP}1p19-positive sera. {C}onclusion: {T}hese data show that naturally acquired {P}. falciparum {MSP}1p19 antibodies are potent inducers of neutrophil {ADRB} and support the development of {P}f{MSP}1p19-based malaria vaccine using {ADRB} assay as a functional surrogate for protection.},
  keywords = {{M}alaria ; {P}lasmodium falciparum ; {ELISA} ; {I}g{G} ; {F}unctional assay ; {C}hemiluminescence ; {N}eutrophils ; {ADRB} ; {MSP}1p19 ; {S}enegal ; {SENEGAL}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {14},
  numero = {},
  pages = {art. 409},
  ISSN = {1475-2875},
  year = {2015},
  DOI = {10.1186/s12936-015-0935-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065388},
}