Bellafiore Stéphane auteur aut IRD Jougla C. auteur aut IRD Chapuis Elodie auteur aut IRD Besnard G. auteur aut IRD Suong M. auteur aut IRD Vu P. N. auteur aut IRD De Waele D. auteur aut IRD Gantet Pascal auteur aut IRD Thi X. N. auteur aut IRD text journalArticle eng text/pdf born digital access Twenty years ago, the facultative meiotic parthenogenetic root-knot nematode (RKN), Meloidogyne graminicola, was recognised as an important rice pathogen in South Vietnam. Although this country is one of the most important rice exporters worldwide, a comprehensive picture of the occurrence of M. graminicola in Vietnamese rice fields is still not available. Therefore a nematode survey was carried out with the aim of better understanding the geographical distribution, and the pathogenic and genetic variability of the RKN in Vietnam. From the fields surveyed in a range of ecosystems, 21 RKN populations were recovered from infected rice roots. A diagnostic SCAR marker was developed showing that all Vietnamese populations belong to M. graminicola. Furthermore, sequencing of the Internal Transcribed Spacer (ITS) of the rDNA genes confirmed this identification. These populations were then characterised using morphometrics and pathogenicity tests (host plant range diversity, reproduction and virulence diversity) revealing intraspecific variability. We showed that morphometric traits are mainly genetically heritable characters with significant differences among the studied populations. Finally, a distinctive trait signature was found for the populations isolated from the upland rice cultures. All together, our study reveals the prevalence of M. graminicola populations in Vietnamese rice. Further investigations need to be developed to explore the population dynamics and evolutionary history of this species in South East Asia. specialized Intraspecific variability Meloidogyne graminicola Rice SCAR Vietnam VIET NAM 076 080 338 7 471-483 2015 1631-0691 https://www.documentation.ird.fr/hor/fdi:010064807 10.1016/j.crvi.2015.04.002 1631-0691 [F B010064807] https://www.documentation.ird.fr/hor/fdi:010064807 https://www.documentation.ird.fr/intranet/publi/2015/08/010064807.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-09-04 2017-08-23 fdi:010064807 fre