Addou N. A. auteur aut IRD Schumann P. auteur aut IRD Sproer C. auteur aut IRD Ben Hania Wajdi auteur aut IRD Hacene H. auteur aut IRD Fauque G. auteur aut IRD Cayol J. L. auteur aut IRD Fardeau Marie-Laure auteur aut IRD text journalArticle eng text/pdf born digital access A novel filamentous, endospore-forming, thermophilic and moderately halophilic bacterium designated strain Nari2A(T) was isolated from soil collected from an Algerian salt lake, Chott Melghir. The novel isolate was Gram-staining-positive, aerobic, catalase-negative and oxidase-positive. Optimum growth occurred at 50-55 degrees C, 7-10% (w/v) NaCl and pH 7-8. The strain exhibited 95.4, 95.4 and 95.2% 16S rRNA gene sequence similarity to Thalassobacillus devorans G19.1(T), Sediminibacillus halophilus EN8d(T) and Virgibacillus kekensis YIM-kknyl 6(T), respectively. The major menaquinone was MK-7. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were iso-C-15:0 and iso-C-17:0. The DNA G+C content was 41.9 molok. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain Nari2A(T) is considered to represent a novel species of a new genus in the family Bacillaceae, order Bacillales, for which the name Melghiribacillus thermohalophilus gen. nov., sp. nov. is proposed. The type strain of Melghiribacillus thermohalophilus is Nari2A(T) (=DSM 25894(T)=CCUG 62543(T)). specialized ALGERIE 084 65 4 1172-1179 2015 1466-5026 https://www.documentation.ird.fr/hor/fdi:010064740 10.1099/ijs.0.000075 1466-5026 [F B010064740] https://www.documentation.ird.fr/hor/fdi:010064740 https://www.documentation.ird.fr/intranet/publi/2015/07/010064740.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-07-29 2017-08-23 fdi:010064740 fre