@article{fdi:010064602, title = {{B}iochemical characterization of ecuadorian wild {S}olanum lycopersicum mill. plants produced from non-cryopreserved and cryopreserved seeds}, author = {{Z}evallos, {B}. and {C}ejas, {I}. and {R}odriguez, {R}. {C}. and {Y}abor, {L}. and {A}ragon, {C}. and {G}onzalez, {J}. and {E}ngelmann, {F}lorent and {M}artinez, {M}. {E}. and {L}orenzo, {J}. {C}.}, editor = {}, language = {{ENG}}, abstract = {{T}his paper presents some of the effects of cryopreservation of wild {S}olanum lycopersicum {M}ill. seeds on the early stages of germination post liquid nitrogen exposure. {P}ercentage of germination, conversion into plantlets and plant fresh mass were evaluated after cryostorage. {L}evels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and proteins were determined. {P}eroxidase and superoxide dismutase activities were recorded. {L}iquid nitrogen exposure increased the percentage of seed germination at 5 days but at 7 days, the conversion into plantlets and the plant fresh mass were not statistically different between non-cryopreserved and cryopreserved samples. {S}everal significant effects of cryopreservation were recorded at the biochemical level at 7 days of germination under controlled conditions. {H}ighly significant effects due to liquid nitrogen exposure were observed in leaves: increased levels of peroxidase enzymatic and specific activities and cell wall-linked phenolics. {V}ery remarkable effects were also recorded in roots: decreased contents of chlorophylls and cell wall-linked phenolics.}, keywords = {tomato ; cryostorage ; biochemical changes ; phenotypic variation ; {EQUATEUR}}, booktitle = {}, journal = {{C}ryo{L}etters}, volume = {34}, numero = {4}, pages = {413--421}, ISSN = {0143-2044}, year = {2013}, URL = {https://www.documentation.ird.fr/hor/fdi:010064602}, }