VanBuren R. auteur aut IRD Zeng F. C. auteur aut IRD Chen C. X. auteur aut IRD Zhang J. S. auteur aut IRD Wai C. M. auteur aut IRD Han J. auteur aut IRD Aryal R. auteur aut IRD Gschwend A. R. auteur aut IRD Wang J. P. auteur aut IRD Na J. K. auteur aut IRD Huang L. X. auteur aut IRD Zhang L. M. auteur aut IRD Miao W. J. auteur aut IRD Gou J. Q. auteur aut IRD Arro J. auteur aut IRD Guyot Romain auteur aut IRD Moore R. C. auteur aut IRD Wang M. L. auteur aut IRD Zee F. auteur aut IRD Charlesworth D. auteur aut IRD Moore P. H. auteur aut IRD Yu Q. Y. auteur aut IRD Ming R. auteur aut IRD text journalArticle eng text/pdf born digital access Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XYh). The hermaphrodite-specific region of the Yh chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Y-h regions of 12 cultivated hermaphrodites. The MSY and HSY regions have highly similar gene content and structure, and only 0.4% sequence divergence. The MSY sequences from wild males include three distinct haplotypes, associated with the populations' geographic locations, but gene flow is detected for other genomic regions. The Y-h sequence is highly similar to one Y haplotype (MSY3) found only in wild dioecious populations from the north Pacific region of Costa Rica. The low MSY3-Y-h divergence supports the hypothesis that hermaphrodite papaya is a product of human domestication. We estimate that Y-h arose only similar to 4000 yr ago, well after crop plant domestication in Mesoamerica >6200 yr ago but coinciding with the rise of the Maya civilization. The Y-h chromosome has lower nucleotide diversity than the Y, or the genome regions that are not fully sex-linked, consistent with a domestication bottleneck. The identification of the ancestral MSY3 haplotype will expedite investigation of the mutation leading to the domestication of the hermaphrodite Y-h chromosome. In turn, this mutation should identify the gene that was affected by the carpel-suppressing mutation that was involved in the evolution of males. specialized AMERIQUE CENTRALE COSTA RICA 076 020 25 4 524-533 2015 1088-9051 https://www.documentation.ird.fr/hor/fdi:010064149 10.1101/gr.183905.114 1088-9051 [F B010064149] https://www.documentation.ird.fr/hor/fdi:010064149 https://www.documentation.ird.fr/intranet/publi/2015/05/010064149.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-06-04 2017-08-23 fdi:010064149 fre