Talamond Pascale auteur aut IRD Verdeil J. L. auteur aut IRD Conejero G. auteur aut IRD text journalArticle eng text/pdf born digital access Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. specialized 020 076 20 3 5024-5037 2015 1420-3049 https://www.documentation.ird.fr/hor/fdi:010064131 10.3390/molecules20035024 1420-3049 [F B010064131] https://www.documentation.ird.fr/hor/fdi:010064131 https://www.documentation.ird.fr/intranet/publi/2015/05/010064131.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-06-04 2020-05-30 fdi:010064131 fre