@article{fdi:010064112,
  title = {{I}nduction of defensin response to dengue infection in {A}edes aegypti},
  author = {{W}asinpiyamongkol, {L}. and {M}iss{\'e}, {D}oroth{\'e}e and {L}uplertlop, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}nnate immune-related defensin peptide expression, the major antimicrobial peptide ({AMP}) in the dengue vector mosquito {A}edes aegypti, was analyzed following infection by dengue virus type 2 (strain 16681) ({DENV}-2). {I}n vitro, the mosquito cell line {C}6/36 was exposed to dengue virus at different multiplicities of infection ({MOI}) in an hour; it was found that the expression level of defensin transcripts was dependent upon viral dose. {I}n addition, using both polymerase chain reaction ({PCR}) and {W}estern blotting assays to determine defensin transcription and translation at a series of times post-inoculation, we found that the time course of defensin transcripts correlated with the expression of defensin peptide post-infection. {I}n vitro, female {A}e. aegypti mosquitoes were fed different meals ({DENV}-2 infected blood, non-infectious blood and sugar) at 14 days post-feeding, to determine defensin transcription in response to dengue infection, by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry ({SELDI}-{TOF}-{MS}) assay. {T}he results showed that a peak of 4.25k{D}a peptide, composed of defensin, was predominantly induced by {DENV}-2 infected blood-fed mosquitoes. {W}e observed that {DENV}-2 could trigger the defensin peptide, defined molecularly as an innate immune response. {T}herefore, this peptide may be involved in dengue infection and/or transmission. {I}mproved understanding of the mosquito's responses to dengue virus should strengthen our understanding of this vector's innate immune system.},
  keywords = {antimicrobial peptides ; {A}ntivirus defense mechanisms ; innate immune response},
  booktitle = {},
  journal = {{E}ntomological {S}cience},
  volume = {18},
  numero = {2},
  pages = {199--206},
  ISSN = {1343-8786},
  year = {2015},
  DOI = {10.1111/ens.12108},
  URL = {https://www.documentation.ird.fr/hor/fdi:010064112},
}