Coudert Y. auteur aut IRD Le V. A. T. auteur aut IRD Adam Hélène auteur aut IRD Bes M. auteur aut IRD Vignols Florence auteur aut IRD Jouannic Stefan auteur aut IRD Guiderdoni E. auteur aut IRD Gantet Pascal auteur aut IRD text journalArticle eng text/pdf born digital access In monocotyledons, the root system is mostly composed of postembryonic shoot-borne roots called crown roots. In rice (Oryza sativa), auxin promotes crown root initiation via the LOB-domain transcription factor (LBD) transcription factor CROWN ROOTLESS1 (CRL1); however, the gene regulatory network downstream of CRL1 remains largely unknown. We tested CRL1 transcriptional activity in yeast and in planta, identified CRL1-regulated genes using an inducible gene expression system and a transcriptome analysis, and used in situ hybridization to demonstrate coexpression of a sample of CRL1-regulated genes with CRL1 in crown root primordia. We show that CRL1 positively regulates 277 genes, including key genes involved in meristem patterning (such as QUIESCENT-CENTER SPECIFIC HOMEOBOX; QHB), cell proliferation and hormone homeostasis. Many genes are homologous to Arabidopsis genes involved in lateral root formation, but about a quarter are rice-specific. Our study reveals that several genes acting downstream of LBD transcription factors controlling postembryonic root formation are conserved between monocots and dicots. It also provides evidence that specific genes are involved in the formation of shoot-derived roots in rice. specialized adventitious root crown root CROWN ROOTLESS1 (CRL1) gene regulatory network LOB-domain transcription factor (LBD) postembryonic root rice (Oryza sativa) 076 206 1 243-254 2015 0028-646X https://www.documentation.ird.fr/hor/fdi:010064013 10.1111/nph.13196 0028-646X [F B010064013] https://www.documentation.ird.fr/hor/fdi:010064013 https://www.documentation.ird.fr/intranet/publi/2015/04/010064013.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-05-04 2017-08-23 fdi:010064013 fre