Ekchariyawat P. auteur aut IRD Hamel Rodolphe auteur aut IRD Bernard E. auteur aut IRD Wichit S. auteur aut IRD Surasombatpattana P. auteur aut IRD Talignani L. auteur aut IRD Thomas F. auteur aut IRD Choumet V. auteur aut IRD Yssel H. auteur aut IRD Desprès P. auteur aut IRD Briant L. auteur aut IRD Missé Dorothée auteur aut IRD text journalArticle eng text/pdf born digital access Arboviruses represent an emerging threat to human. They are transmitted to vertebrates by the bite of infected arthropods. Early transmission to vertebrates is initiated by skin puncture and deposition of virus in this organ. However, events at the bite site remain largely unknown. Here, we report that Chikungunya virus (CHIKV) and West Nile virus (WNV), despite belonging to distinct viral families, elicit a common antiviral signature in primary human dermal fibroblasts, attesting for the up regulation of interferon signaling pathways and leading to an increased expression of IFN-β, interleukins and chemokines. Remarkably, CHIKV and WNV enhance IL-1β expression and induce maturation of caspase-1, indicating the capacity of these pathogens to elicit activation of the inflammasome program in resident skin cells. CHIKV and WNV also induce the expression of the inflammasome sensor AIM2 in dermal fibroblasts, whereas inhibition of caspase-1 and AIM2 with siRNA interferes with both CHIKV- and WNV-induced IL-1β production by these cells. Finally, inhibition of the inflammasome via caspase-1 silencing was found to enhance CHIKV replication in dermal fibroblasts. Together, these results indicate that the skin contributes to the pro-inflammatory and anti-viral microenvironment via the activation of the inflammasome in the early stages following infection with arboviruses. specialized ARBOVIROSE MOUSTIQUE INFECTION MECANISME DE DEFENSE IMMUNITE GENE ARN PROTEINE REPONSE IMMUNITAIRE EXPRESSION DES GENES PEAU 052CULARB03 32 401-408 2015 1567-1348 https://www.documentation.ird.fr/hor/fdi:010063775 10.1016/j.meegid.2015.03.025 1567-1348 [F B010063775] https://www.documentation.ird.fr/hor/fdi:010063775 https://www.documentation.ird.fr/intranet/publi/depot/2015-04-29/010063775.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-04-28 2015-09-28 fdi:010063775 fre