Insecticidal activities of bark, leaf and seed extracts of Zanthoxylum heitzii against the african malaria vector Anopheles gambiae Overgaard H. J. auteur aut IRD Sirisopa P. auteur aut IRD Mikolo B. auteur aut IRD Malterud K. E. auteur aut IRD Wangensteen H. auteur aut IRD Zou Y. F. auteur aut IRD Paulsen B. S. auteur aut IRD Massamba D. auteur aut IRD Duchon Stéphane auteur aut IRD Corbel Vincent auteur aut IRD Chandre Fabrice auteur aut IRD text journalArticle eng text/pdf born digital access The olon tree, Zanthoxylum heitzii (syn. Fagara heitzii) is commonly found in the central-west African forests. In the Republic of Congo (Congo-Brazzaville) its bark is anecdotally reported to provide human protection against fleas. Here we assess the insecticidal activities of Z. heitzii stem bark, seed and leaf extracts against Anopheles gambiae s.s, the main malaria vector in Africa. Extracts were obtained by Accelerated Solvent Extraction (ASE) using solvents of different polarity and by classical Soxhlet extraction using hexane as solvent. The insecticidal effects of the crude extracts were evaluated using topical applications of insecticides on mosquitoes of a susceptible reference strain (Kisumu [Kis]), a strain homozygous for the L1014F kdr mutation (kdrKis), and a strain homozygous for the G119S Ace1R allele (AcerKis). The insecticidal activities were measured using LD50 and LD95 and active extracts were characterized by NMR spectroscopy and HPLC chromatography. Results show that the ASE hexane stem bark extract was the most effective compound against An. gambiae (LD50 = 102 ng/mg female), but was not as effective as common synthetic insecticides. Overall, there was no significant difference between the responses of the three mosquito strains to Z. heitzii extracts, indicating no cross resistance with conventional pesticides. specialized Zanthoxylum heitzii Anopheles malaria bark extract insecticide CONGO AFRIQUE DE L'OUEST AFRIQUE CENTRALE 052 076 Molecules 19 12 21276-21290 2014 1420-3049 https://www.documentation.ird.fr/hor/fdi:010063655 10.3390/molecules191221276 1420-3049 [F B010063655] https://www.documentation.ird.fr/hor/fdi:010063655 https://www.documentation.ird.fr/intranet/publi/2015/02/010063655.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-03-03 2017-08-23 fdi:010063655 fre