Ben Aissa F. auteur aut IRD Postec A. auteur aut IRD Erauso G. auteur aut IRD Payri Claude auteur aut IRD Pelletier Bernard auteur aut IRD Hamdi M. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Ollivier Bernard auteur aut IRD text journalArticle eng text/pdf born digital access A novel anaerobic, alkaliphilic, Gram-positive staining bacterium was isolated from a hydrothermal chimney in the Prony Bay, New Caledonia. This strain designated FatMR1(T) grew at temperatures from 20 to 55 A degrees C (optimum 37 A degrees C) and at pH between 7.5 and 10.5 (optimum 8.8-9). NaCl is not required for growth (optimum 0.2-0.5 %), but is tolerated up to 3 %. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite are not used as terminal electron acceptors. Strain FatMR1(T) fermented pyruvate, yeast extract, peptone and biotrypcase and used fructose as the only sugar. The main fermentation products from fructose and proteinaceous compounds (e.g. peptone and biotrypcase) were acetate, H-2 and CO2. Crotonate was disproportionated to acetate and butyrate. The predominant cellular fatty acids were C-14:0 and C-16:0. The G + C content of the genomic DNA was 37.1 mol %. On the basis of phylogenetic, genetic, and physiological properties, strain FatMR1(T) (=DSM 25890(T), =JCM 18390(T)) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as a novel species of the genus Alkaliphilus, A. hydrothermalis sp. nov. specialized Firmicutes Alkaliphilus hydrothermalis Alkaliphilic Anaerobic Alkaline hydrothermal vents Serpentinization New Caledonia NOUVELLE CALEDONIE 036 084 19 1 183-188 2015 1431-0651 https://www.documentation.ird.fr/hor/fdi:010063622 10.1007/s00792-014-0697-y 1431-0651 [F B010063622] https://www.documentation.ird.fr/hor/fdi:010063622 https://www.documentation.ird.fr/intranet/publi/2015/01/010063622.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-02-02 2017-08-23 fdi:010063622 fre