Bernard E. auteur aut IRD Hamel Rodolphe auteur aut IRD Neyret A. auteur aut IRD Ekchariyawat P. auteur aut IRD Molès J.P. auteur aut IRD Simmons G. auteur aut IRD Chazal N. auteur aut IRD Desprès P. auteur aut IRD Missé Dorothée auteur aut IRD Briant L. auteur aut IRD text journalArticle eng text/pdf born digital access Transmission of chikungunya virus (CHIKV) to humans is initiated by puncture of the skin by a blood-feeding Aedes mosquito. Despite the growing knowledge accumulated on CHIKV, the interplay between skin cells and CHIKV following inoculation still remains unclear. In this study we questioned the behavior of human keratinocytes, the predominant cell population in the skin, following viral challenge. We report that CHIKV rapidly elicits an innate immune response in these cells leading to the enhanced transcription of type I/II and type III interferon genes. Concomitantly, we show that despite viral particles internalization into Rab5-positive endosomes and efficient fusion of virus and cell membranes, keratinocytes poorly replicate CHIKV as attested by absence of nonstructural proteins and genomic RNA synthesis. Accordingly, human keratinocytes behave as an antiviral defense against CHIKV infection rather than as a primary targets for initial replication. This picture significantly differs from that reported for Dengue and West Nile mosquito-borne viruses. specialized ARBOVIROSE TRANSMISSION INFECTION BIOLOGIE MOLECULAIRE VECTEUR MOUSTIQUE IMMUNITE ANTIGENE CHIKUNGUNYA PEAU KERATINOCYTE 052CULARB03 476 1-10 2015 0042-6822 https://www.documentation.ird.fr/hor/fdi:010063328 10.1016/j.virol.2014.11.013 0042-6822 [F B010063328] https://www.documentation.ird.fr/hor/fdi:010063328 https://www.documentation.ird.fr/intranet/publi/depot/2015-02-10/010063328.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-02-10 2019-06-07 fdi:010063328 fre