Quéméneur Marianne auteur aut IRD Bes M. auteur aut IRD Postec A. auteur aut IRD Mei N. auteur aut IRD Hamelin J. auteur aut IRD Monnin C. auteur aut IRD Chavagnac V. auteur aut IRD Payri Claude auteur aut IRD Pelletier Bernard auteur aut IRD Guentas-Dombrowsky Linda auteur aut IRD Gérard Martine auteur aut IRD Pisapia C. auteur aut IRD Gérard E. auteur aut IRD Ménez B. auteur aut IRD Ollivier Bernard auteur aut IRD Erauso G. auteur aut IRD text journalArticle eng text/pdf born digital access The shallow submarine hydrothermal field of the Prony Bay (New Caledonia) discharges hydrogen- and methane-rich fluids with low salinity, temperature (< 40°C) and high pH (11) produced by the serpentinization reactions of the ultramafic basement into the lagoon seawater. They are responsible for the formation of carbonate chimneys at the lagoon seafloor. Capillary electrophoresis single-strand conformation polymorphism fingerprinting, quantitative polymerase chain reaction and sequence analysis of 16S rRNA genes revealed changes in microbial community structure, abundance and diversity depending on the location, water depth, and structure of the carbonate chimneys. The low archaeal diversity was dominated by few uncultured Methanosarcinales similar to those found in other serpentinization-driven submarine and subterrestrial ecosystems (e.g. Lost City, The Cedars). The most abundant and diverse bacterial communities were mainly composed of Chloroflexi, Deinococcus-Thermus, Firmicutes and Proteobacteria. Functional gene analysis revealed similar abundance and diversity of both Methanosarcinales methanoarchaea, and Desulfovibrionales and Desulfobacterales sulfate-reducers in the studied sites. Molecular studies suggest that redox reactions involving hydrogen, methane and sulfur compounds (e.g. sulfate) are the energy driving forces of the microbial communities inhabiting the Prony hydrothermal system. specialized LAGON MILIEU RECIFAL FOND MARIN ROCHE ULTRABASIQUE FAUNE BENTHIQUE MICROORGANISME STRUCTURE DU PEUPLEMENT ABONDANCE DIVERSITE SPECIFIQUE PROFONDEUR PHYLOGENIE FAUNE HYDROTHERMALE NOUVELLE CALEDONIE PACIFIQUE 036MILMAR 084FERMEN02 6 6 665–674 2014 1758-2229 https://www.documentation.ird.fr/hor/fdi:010062924 10.1111/1758-2229.12184 1758-2229 [F B010062924] https://www.documentation.ird.fr/hor/fdi:010062924 https://www.documentation.ird.fr/intranet/publi/depot/2014-10-10/010062924.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-10-10 2015-02-03 fdi:010062924 fre