@article{fdi:010062657,
  title = {{C}omparative analysis of {I}g{G} responses to {P}lasmodium falciparum {MSP}1p19 and {PF}13-{DBL}1 alpha 1 using {ELISA} and a magnetic bead-based duplex assay ({MAGPIX} ({R})-{L}uminex) in a {S}enegalese meso-endemic community},
  author = {{P}erraut, {R}. and {R}ichard, {V}. and {V}arela, {M}. {L}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {G}uillotte, {M}. and {T}all, {A}. and {T}oure, {A}. and {S}okhna, {C}heikh and {V}igan-{W}omas, {I}. and {M}ercereau-{P}uijalon, {O}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {N}umerous {P}lasmodium falciparum antigens elicit humoral responses in humans living in endemic areas. {U}se of multiplex assays is a convenient approach to monitor the antibody response against multiple antigens, but to integrate multiplex assay-derived data with datasets, generated previously using {ELISA}, comparative studies are needed. {T}his work compares antibody responses to two {P}. falciparum antigens monitored using both technologies. {M}ethods: {T}he {I}g{G} response against the merozoite surface protein-1 {P}f{MSP}1p19 and the {PF}13-{DBL}1 alpha 1 domain of the {P}. falciparum {E}rythrocyte {M}embrane {P}rotein1, expressed by the rosette-forming parasite 3{D}7/{PF}13 ({PF}13), was investigated using {ELISA} and a {MAGPIX} ({R})-{L}uminex duplex assay. {A}rchived plasma samples collected before the rainy season from 217 villagers living in {N}diop, a {S}enegalese meso-endemic setting, were studied. {ROC} analysis was used to define the optimal antibody measure readout. {A}ssociation of antibody levels with protection against clinical malaria was analysed using {P}oisson regression in a retrospective study from active case detection records performed during the 5.5-month transmission season that followed blood sampling. {R}esults: {T}here was a strong positive correlation ({P} <10(-3)) between {ELISA} and {MAGPIX} ({R})-{L}uminex-{MFI} (median fluorescence intensity) values for antibody to {P}f{MSP}1p19 (rho = 0.78) and {PF}13-{DBL}1a1 (rho = 0.89), with a similar degree of concordance in all age groups. {A}ntibody levels to both antigens were high but displayed a different age-associated pattern. {I}ndependent age-adjusted {P}oisson regression analysis showed a significant association with protection only for {I}g{G} responses to {MSP}1p19 ({P} <0.01 {RR} = 0.71 [0.53-0.93]) measured by {ELISA}. {C}onclusion: {T}he individual {ELISA} and duplex-{MAGPIX} assays provide a concordant evaluation of age-associated antibody responses to {MSP}1p19 and {PF}13-{DBL}1a1, irrespective of the formulation of antibody levels (values, ratios or {ROC}-adjusted figures) but do diverge with regard to the association of antibody levels with clinical protection in age-adjusted models. {T}his may reflect incomplete overlap of the epitopes presented in the two formats. {F}urther development for multiplex assessment of antibody responses to a larger panel of antigens with the robust and cost effective {MAGPIX} ({R})-{L}uminex technology is warranted.},
  keywords = {{M}alaria ; {P}lasmodium falciparum ; {ELISA} ; {I}g{G} ; {S}urface antigens ; {MSP}1p19 ; {P}f{EMP}1-{PF}13 ; {M}ultiplex ; {MAGPIX} ; {S}enegal ; {N}diop ; {SENEGAL}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {13},
  numero = {},
  pages = {410},
  ISSN = {1475-2875},
  year = {2014},
  DOI = {10.1186/1475-2875-13-410},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062657},
}